Optimizing PFAS Analysis via Draft Method 1633 Using a Stacked SPE Format
Date: 25. May 2023
Time: 10:00 - 11:00 (BST) | 11:00 - 12:00 (CEST)
Key Learning Points:
Learn options for SPE phases for PFAS extraction from water and soil samples.
Utilize acceptable options for EPA 1633 as a guidance method.
Understand how to optimize time and cost for sample preparation.
Event Overview
This webinar will present data and discuss EPA Draft Method 1633 compliant options to simplify sample clean up and increase sample throughput for PFAS analysis.
Presenters: Presenters: Richard Jack, Global Market Development Manager, Food and Environmental, Phenomenex & Philip Bassignani, Senior Director of Research and Development, Alpha Analytical
SEC-nHRMS for the Characterization of mAbs
Date: 15. February 2023
Time: 10:00 - 11:00 (GMT) | 11:00 - 12:00 (CET)
Key Learning Points:
Receive a demonstration of how non-denaturing SEC-Native MS can be leveraged to streamline mAb characterization, including methods for the digestion of mAbs.
Listen in to a column selection discussion, which includes pore volume and column dimensions to ensure monomer and aggregate resolution, and optimize mobile phases for complex characterizations.
Learn about SEC combined with nHRMS, and how to select a SEC column.
Who should attend?
Any scientists wanting to learn how to combine their size exclusion chromatography and High-resolution Mass Spec for improvements in their mAb characertizations.
Presenter: Lorne Nelson
Senior Scientist - Field Applications, Phenomenex
HPLC Toubleshooting
Date: 15. June 2023
Time: 10:00 - 11:00 (BST) | 11:00 - 12:00 (CEST)
Key Learning Points:
Learn how to prevent and minimize problems before they occur.
Understand how to approach problem solving and troubleshooting in a logical way, including: diagnose cause and possible solutions.
Review peak shape, and column performance problems.
Who should attend?
Any LC users who would like to get tips on extend the column lifetime.
Presenter: Kevin Zhao, Senior Technical Specialist, Phenomenex
Chiral Chromatography Screening 101
Date: 22. June 2023
Time: 10:15 - 11:15 (BST) | 11:15 - 12:15 (CEST)
Key Learning Points:
Learn why chiral screening is done, what are the benefits.
Take a deep dive into columns, and screening modes/methods.
Listen into sample compatibility considerations including: detection, solubility, and chiral centers.
Understand quick, easy, and cost-effective ways to identify a solution for your chiral separation needs.
Who should attend?
Any LC users who would like to get tips on a chiral screening process for method development.
Any academia or pharmaceutical scientists who need to rapidly develop a method for separation and characterization of their chiral compounds.
Practical Approaches for Identifying Equivalent and Orthogonal Columns
Date: 27. June 2023
Time: 10:00 - 11:00 (BST) | 11:00 - 12:00 (CEST)
Key Learning Points:
The selection of a suitable column for HPLC and UHPLC method development can be challenging. There is an immense number of columns to choose from, with almost 400 containing the ever-popular C18 phase alone and an ever-expanding offering of non-C18 stationary phases being introduced and marketed for everything from improved retention for polar analytes to improved peak shape for basic analytes. Columns that offer very similar selectivity can provide an alternative, or back up column, for a method. Columns having different selectivities may be desired when alternative, or orthogonal, separations are necessary, or when different elution orders are beneficial for complex separations. In this lecture we will review several different strategies for classifying and identifying similar and very different columns.
Identifying columns with similar selectivity to your current column of choice.
Identifying columns with different or orthogonal selectivity to your current column of choice.
Understanding the resources that are available for selectivity data for HPLC and UHPLC columns.
Who should attend?
Any LC users, in particular those who are working on SM Pharma and Generic Pharma.
Presenter: Heiko Behr, Senior Business Development Manager, Phenomenex
PFAS EPA Method Development and Findings for UCMR5
Date: 29. June 2023
Time: 10:00 - 10:00 (BST) | 11:00 - 12:00 (CEST)
Abstract:
The U.S. EPA, Office of Ground Water and Drinking Water, Technical Support Branch develops drinking water analytical methods supporting programs, such as the Unregulated Contaminant Monitoring Rule (UCMR) Program. The UCMR Program is a national occurrence study used to collect data for contaminants that are suspected to be present in drinking water and do not have health-based standards set under the Safe Drinking Water Act (SDWA). Rugged analytical methods are needed to quantify these contaminants, meaning the methods must yield reliable, accurate results over the range of field and lab conditions specified in the method. To ensure that analytical methods meet these criteria, EPA develops drinking water methods using a rigorous validation process that assesses the method performance under various conditions that would support method use at a national level. This validation process includes interference and contamination assessments, adherence to quality control acceptance criteria, quantitation limit determinations, sample preservation efficacies, and multi-laboratory comparisons. Per- and polyfluoroalkyl substances (PFAS) constitute a significant portion of the fifth UCMR cycle (UCMR 5).
In this webinar, William Adams, PhD (EPA) will give an overview of EPA analytical method development and validation processes for drinking water methods supporting the UCMR Program, using EPA published and in-development PFAS analytical methods as examples.
Agustin Pierri (Weck Laboratories) will add the experiences of a commercial laboratory doing PFAS analysis both in support of UCMR5 and for routine drinking water compliance monitoring. Weck Laboratories has been analyzing environmental samples for PFAS for over fifteen years and has developed a comprehensive understanding of the challenges associated with this type of analysis. In the first few months of UCMR5 testing and the first years of routine compliance monitoring in California, the lab has seen some interesting results. Dr. Pierri will cover the differences between the commonly used analytical methods and their applications, some of the challenges that may arise with each method, as well as the results and what they could mean for water quality. Suggestions on how to overcome these challenges and how to successfully deploy these methods in a commercial laboratory will also be examined.
Key Learning Points:
Learn how the EPA Office of Ground Water and Drinking water optimizes and validates methods for UCMR5.
Understand how one lab is applying this method to determine PFAS in real water samples.
Find out what LODs and LOQs are seeing in the first six months of UCMR5.
Who should attend?
Anyone interested in PFAS contamination in drinking water and looking to sort out analytical challenges. .
Presenters: William A. Adams, Chemist, U.S. EPA
& Agustin Pierri, Laboratory Technical Director, Weck Labs
Rajesh Babu Dandamudi, PhD
Rajesh Babu has more than 11 years of chromatography experience and is an expert in practical HPLC/UHPLC/LC-MS/GC-MS method development and troubleshooting. Before joining Phenomenex, Dr. Rajesh worked as a Faculty member at Sri Sathya Sai Institute of Higher Learning, Deemed University. He was instrumental in establishing a state-of-the-art mass-spec core facility consisting of GC, HPLC, GC-MS, LC-MS/MS, and HRMS systems. His research work was in the area of clinical metabolomics.
Margarucci Luigi, PhD
Luigi, PhD in Pharmaceutical Sciences, has been working with Phenomenex since 2015 and currently holds the position of Technical Manager. He gained experience in the field of chromatography coupled to mass spectrometry working as a researcher/assistant at the University of Salerno and collaborating with important reference centres for chromatography and mass spectrometry, in Italy and Europe (University of Utrecht - NL). To date, he is the author of 27 scientific publications. In 2020, he became responsible for chromatography training for our customers, and from 2021 he took on the role of Technical Manager for Italy.
Mackenzie Freige
Mackenzie is an Application Scientist at PhenoLogix, and is based in Torrance, CA. She has undergraduate degrees in Chemistry and in Life Sciences. She joined Phenomenex in 2021, and brings extensive experience in liquid chromatography-mass spectrometry. In her free time she practices yoga and enjoys reading.
James Turner
After graduating from UMIST with a degree in Chemistry I then studied for an MSC in Instrumentation and Analytical Science. I then worked with another HPLC column manufacturer before joining Phenomenex in 1996. With more than 25 years of experience at Phenomenex, I have been responsible for providing specialist technical support to customers across Europe with method development assistance, troubleshooting advice and chromatographic solutions to various problems.
Genevieve Hodson
Genevieve graduated from the University of Texas with a BS in Chemistry located in her home town of Austin Texas. She spent 2 years of her undergraduate performing research in an Organic Chemistry Lab synthesizing intermediates. Out of school she spent 4 years as an Analytical Chemist in the QC and R&D Department of Cerilliant (a Sigma Millipore Company). There she worked with many analytical instruments but became an expert at LC by performing purity analysis, customer specific methods, validations, qc testing and stability studies on a wide range of small molecules including pharmaceuticals, pesticides, explosives and illegal substances. From there she moved to Los Angeles California where she worked at Johnson and Johnson and then a small dietary supplement company. After Genevieve spent a year living abroad in Israel raising her first son, she moved back to LA where she got hired as a Technical Specialist at Phenomenex. At Phenomenex she enjoys continuing to learn about chromatography every day through helping customer with all their chromatography needs
Helen Whitby, PhD
Dr. Helen Whitby is the senior product manager for biopharma at Phenomenex. Helen has been with Phenomenex since 2007, starting in the UK sales team as a bulk and prep specialist before joining the technical team in 2016. Helen spent 6 years as a biopharma specialist in the technical team supporting all our biopharma products globally before moving to product management in 2022. Helen has a PhD from the University of Manchester working on analogues of Shikimic acid to treat malaria. Prior to joining Phenomenex, Helen spent a year as a Post-doctoral researcher at the University of Illinois in Chicago, where she studied electron-deficient divalent reactive intermediates, specifically nitrenium ions for the development of antiviral agents to treat Ebola.
Rajesh Babu Dandamudi, PhD
Rajesh Babu has more than 11 years of chromatography experience and is an expert in practical HPLC/UHPLC/LC-MS/GC-MS method development and troubleshooting. Before joining Phenomenex, Dr. Rajesh worked as a Faculty member at Sri Sathya Sai Institute of Higher Learning, Deemed University. He was instrumental in establishing a state-of-the-art mass-spec core facility consisting of GC, HPLC, GC-MS, LC-MS/MS, and HRMS systems. His research work was in the area of clinical metabolomics.
Eduardo Morales
Eduardo has worked at Weck Laboratories for over 20 years. During this time, he has worked exclusively in the organic lab where he has been involved in all aspects of the operation. He now serves as a supervisor of organic chemistry where he is responsible for the development of new analytical methods, optimization of extraction techniques, mostly for emergent contaminants, and the development and implementation of protocols throughout the lab. Mr. Morales holds a Bachelor of Science in biochemistry from California State University Los Angeles
Philip Leong
Philip Leong has worked in the biotechnology industry for over 16 years with Life Science and Clinical Diagnostics background. He received his Bachelor’s degree in Bioengineering from UC San Diego and a Master’s degree in Biotechnology from California State University, Fullerton. He has held roles in Technical Support both in Life Science and Diagnostics, as well as roles in Product Management and Global Marketing. He joined Phenomenex in July of 2021 and is the Global Product Manager for SPE Sample Preparation. Outside of work, Philip enjoys traveling, running in long distance races, and exploring different cuisines from around the world.
Kevin Zhao
Kevin Zhao is the Senior Technical Specialist with Phenomenex and has 5 years of experience in biochemistry research; 4 years of Protein/Cancer research with CSULA-City of Hope Cancer Collaborative and over 9 years in Phenomenex. Mr. Zhao is a member of a global Technical Support Team which includes providing problem-solving, troubleshooting, and applications assistance for customers worldwide in the fields of chromatography (liquid and gas) and sample preparation, and also handling Industries supported include – pharmaceutical and biopharmaceutical, clinical research and clinical toxicology, food safety, environmental, fuels and specialty chemicals. Mr. Zhao received his B.S. in Biology at the California State Polytechnic University, Pomona. earned his M.S. in Biochemistry at California State University, Los Angeles, and also earned a MBA at the University of the Incarnate Word.
Sergio Guazzotti. PhD
Sergio Guazzotti is the Business Development Manager for Micro and Nano LC for Phenomenex in Europe. Prior to this role, Sergio worked at Thermo Fisher Scientific for 11 years in the Chromatography and Mass Spectrometry Division, covering areas of HPLC, LC-MS, GC/ GC-MS, IC, and IC-MS. Sergio obtained his Master and Ph.D. in Chemistry from the University of California. Earlier in his career Sergio was faculty at the University of California, San Diego in La Jolla, CA and held several positions at Nanostream Inc. in Pasadena, CA.
Shahana W. Huq
Shahana W. Huq is a Senior Application Scientist focusing on Clinical Research and Sample Preparation, within the PhenoLogix applications lab at Phenomenex. She holds a MS in Biochemistry from California State University, Los Angeles. Her work involves analytical method development, optimization and troubleshooting. She has experience in R&D product and application development as well as extensive hands on experience in sample preparation and LC-MS/MS. She works closely with customers in the clinical research industry, providing technical support and consultation as well as webinars and onsite seminars. She has been working for Phenomenex since the year 2000.
Jeffery Moran, PhD
Jeffery Moran, Ph.D. is an analytical toxicologist with over 25 years of experience in analytical chemistry, metabolomics, and human biomarkers. Currently, Dr. Moran is the CEO of PinPoint Testing, LLC and faculty in the College of Medicine at the University of Arkansas for Medical Sciences. The Mission of PinPoint Testing, LLC is to “Make Analytical Toxicology Easy” through innovative ToxBox® test kits and solutions. Dr. Moran’s career spans many facets that include medicine, public health, environmental, food, and industrial hygiene. His research primarily focuses on the detection and mechanisms of environmental toxicants and xenobiotics. His recent work includes the detection and characterization of synthetic cannabinoids (SCBs), which emerged as a major drug of abuse. Dr. Moran’s passion is creating sustainable solutions for analytical testing laboratories.
Jörg Baute, PhD
Dr. Jörg Baute earned his PhD in organic chemistry at Essen University. He began working for Phenomenex in 2004, starting as a Technical Sales Consultant responsible for HPLC in Germany. In 2005 he was promoted to the outside sales group at Phenomenex and was responsible for the Key Account companies (Pharma and classic Chemistry) in Germany. In 2009, he became European GC and SPE Business Development Manager, with the responsibility for supporting our distributors and their customers across Europe. During this time he was involved in the launch of many new products in HPLC, GC and SPE, including the introduction of Kinetex Core Shell technology in 2009. Since 2014, he has been the European Food and Environmental Business Development Manager, with the responsibility for HPLC, GC and SPE support in these industries. As part of his overall responsibilities he has given hundreds of lectures in Method development and HPLC and GC Trouble Shooting all across Europe. He is also part of our Global Technical Support team for all of our customers in Europe.
Zachary Woodward
Zachary Woodward is a Technical Specialist with Phenomenex, Inc, assisting laboratories with method development in the bio-pharma, environmental, and food industries. Zachary spent his formative years developing HPLC and GC methods with AMRI in Albany, NY, after which he developed HPLC and SPE methods with Focus Diagnostics in Cypress, CA.
Ramkumar Dhandapani, PhD
Ramkumar Dhandapani has been in the chromatography industry for over 17 years with hands-on and troubleshooting experience. He has earned a Master's and Ph.D. degree in Analytical Chemistry from Seton Hall University with specialization in Microextractions, Multidimensional Gas Chromatography, and Tandem Mass Specs techniques. He has developed and validated several regulatory compliant methods in Pharmaceutical, Food, Fuels, and Environmental industry as well as incorporated method improvement and troubleshooting across a range of separation techniques. Dr. Dhandapani joined Phenomenex in August of 2014 and he serves as the Global Product Manager at Phenomenex. In addition to managing the GC product line, Dr. Dhandapani presents innovations in Chromatography at various chromatography conferences.
Ivan Lebedev
Ivan has an extensive and diverse background in varying biologics-focused applications including the analytics of oligonucleotides, monoclonal antibodies, and conventional proteins. He has a PhD in Biochemistry and Structural Biology with a former background in Biochemical Engineering – currently software focused. He’s lived all over both coasts and is always happy to hop on a plane to visit one or the other.
Dr. Heiko Behr
Dr. Heiko Behr is currently European Pharmaceutical Senior Business Development Manager at Phenomenex. He has over 30 years of experience in Chromatography.His primary responsibilities are consulting with Pharmaceutical Scientists to determine the best implementation of new HPLC technologies. He is also giving educational seminars about Method Development, Troubleshooting, System Optimizations etc.. In addition he is responsible for Ph. Eur. Projects at Phenomenex globally. Prior to joining Phenomenex Dr. Behr graduated with a Diplom in Chemistry at University of Kassel and received his PhD in Analytical Chemistry at Technical University of Munich under the advisement of Professor Harun Parlar, with the main focus being Chromatography Theory and Techniques. From 1993 to 1998 he was involved in teaching chromatography techniques to students at the University of Kassel in his function as a scientific assistant of the department Analytical Chemistry. Dr. Behr joined Phenomenex as a Technical Consultant in 1999. Since then he has also been the Senior Product Specialist for HPLC columns in Germany/Austria and Sales & Business Development Manager for European Distributors.
Olivier Drouet
Olivier, holds a Msc.D in Analytical & Organic Chemistry.
After a career outset spent in lab as a scientist working in the pharmaceutical and cosmetics fields, he embraced a new chapter by providing customized technical support & commercial solutions in the field of the analytical, Flash & preparative Chromatography for about 15 years, he has been working at Phenomenex for 3 years as an European Business Development Manager for Purification, Most of his time is devoted to provide solutions for Flash, preparative & Process Chromatography in the domains of chemistry, pharmaceuticals, biotechnology, Food & natural products.
John Gebler
John Gebler is currently the Director of Strategic Biopharma Business Development for Phenomenex. He has been with Phenomenex since 2021 working with Phenomenex and Danaher leadership to create and manage a biopharma separations and sample prep consumables growth vision. John also manages the execution of biopharma roadmap with Phenomenex innovation, marketing, and sales partners. He has his Ph.D. from the University of Utah studying mechanistic aspects of prenyl transferases. In John's spare time, he enjoys time with family, hiking, biking, camping, and traveling.
Swetha Kotikalapudi
Technical Manager, Phenomenex Swetha Kotikalapudi has over 16 years of chromatography experience and is an expert in practical HPLC/UHPLC/LC-MS/GC-MS method development and troubleshooting. Prior to joining Phenomenex, Swetha worked as a Senior Research Associate with DuPont, Hyderabad, where she led efforts in establishing state-of-the-art facility corporate Centre of Analytical Sciences and was involved with multiple HPLC, LC-MS, and GC method development for small molecules, polymers and polysaccharides. Swetha joined Phenomenex in 2018 as the Technical Manager for India and is a member of the global technical team at Phenomenex, USA. In her current role, Swetha provides technical, application development and troubleshooting support to chromatographers worldwide. Swetha has earned a master’s degree in Analytical chemistry from Osmania University, Hyderabad and brings with her vast experience in analytical method development, validations and structural elucidations using NMR for various small molecules.
Grace Guo
Senior Technical Specialist, Phenomenex USA Grace graduated from the Johns Hopkins University with a Master’s degree in Material Science and Engineering in 2014. In her graduate study, she developed a low-voltage organic phototransistor with an organic small molecule. After her graduation she moved from the east coast to west coast because she loves the all year long sunshine in South California. She spent 3 years in a start-up company as Device Scientist doing single walled carbon nanotube separation and making thin film transistor with them. Most of her experience with Flash system are gained from there. In 2017, she joined Phenomenex as a Technical Specialist supporting globally, especially the Asia-Pacific area. Bilingual in Chinese and English makes it easy for her to communicate with customers in both US and China. At Phenomenex she has more opportunity to have hands-on experience in HPLC, GC and SPE. Discussing the crazy troubleshooting cases with other technical members improves her chromatographic skills every day!
Namrata Saxena
Namrata is the Technical Manager for the Asia-Pacific region in Phenomenex and is based in Sydney, Australia. As a member of the global technical team, she is responsible for assisting external customers as well as internal marketing and sales teams of Phenomenex for multifarious technical queries. She specifically support company’s bio-products portfolio through external and internal seminars/webinars, trainings and also contribute in biologics related technical content creation.
Before joining Phenomenex she had worked as a Product Specialist and spent several years in stationary phase chemistry, column manufacturing and method development groups in a German company Bischoff Chromatography GmbH.
Namrata has a post graduate degree with specialization in Analytical Chemistry. Her competence and focus lies in biotherapeutics characterization through liquid chromatography, antibodies and oligonucleotide chemistry, gene therapy and new bio-therapeutic modalities.
Philip Bassignani
Philip Bassignani is the Director of Technical Operations with Alpha Analytical and he has over 25 years of environmental analytical and method development experience, working on a wide range of projects. Philip serves as the laboratory’s emerging contaminants and HRMS technical specialist, with a strong emphasis on PFAS extraction and analytical method development.
Agustin Pierri, Ph.D., Technical Director at Weck Laboratories
Dr. Agustin Pierri obtained his BS in Chemistry from the University of Southern California and his Ph.D. in Chemistry from the University of California Santa Barbara. He has been an analyst in different sections of the laboratory and since 2007 has worked in the LC-MS/MS department developing all analytical methods by that technique. Prior to becoming Technical Director in July of 2016, he has been working as Methods Development Manager for all departments of the Laboratory.
William A. Adams, Ph.D., Chemist U.S. EPA, Office Ground Water and Drinking Water
Dr. Adams is a Chemist with the U.S. EPA, Office of Ground Water and Drinking Water, Technical Support Branch (TSB) in Cincinnati, OH, where he serves as the TSB project officer for EPA drinking water method development, manages the EPA Drinking Water Alternate Test Procedure (ATP) Program, and advises the UCMR program as a technical and analytical method resource. He received a Bachelor of Science in Chemistry and a Ph.D. in Analytical Chemistry from The University of Alabama.
Zandra Baja
Zandra is an Applications Scientist at PhenoLogix. She joined Phenomenex in June of 2021 and brings over 10 years of experience in analytical chemistry.
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