Troubleshoot HPLC: Identifying, Solving and Avoiding Problems

(Course No. SS0-9379)

Date and Location
1-2 July 2025
12:00 - 16:00 GMT | 13:00 - 17:00 CET
Online
or 18-19 November 2025
08:30 - 12:30 GMT | 09:30 - 13:30 CET
Online

Please note: the seminars take place in two half days (2 sessions of 4 hours each).

Price (per person)
£425 | 485 € | 480 CHF | 1200 PLN | 3600 DKK | 5100 NOK | 5350 SEK | 9300 CZK (+VAT, incl. PDF of the slides and certificate of attendance)

Course Summary

Learn how to find solutions to problems encountered when running HPLC analysis by diagnosing symptoms and implementing appropriate preventative measures. This course is ideal for those who have experience using HPLC and now want to develop their skills further.

Course Outline

Problem Solving Strategy:

  • Leaks
  • Variable retention times
  • Quantitation and data quality
  • Baseline
  • System effects

General Chromatographic Problems:

  • Assessing the symptoms
  • Making the diagnosis
  • Finding the appropriate solution
  • System problem, preventive maintenance and column care

Peak Problems:

  • Fronting and tailing
  • Peak split
  • Negative and ghost peaks
  • Real case study

Practical Skills Acquired

This course will enable you to go back to your lab with a full understanding of why problems may arise with your HPLC system and give you the skills and knowledge to both prevent and resolve those problems. In addition you will be able to:

  1. Approach and follow the general steps on HPLC troubleshooting.
  2. Troubleshoot general chromatographic problems, including pressure, leaks, variable retention times, quantitation, data quality and baseline issues.
  3. Identify the most common peak issues and the possible causes.
  4. Clean/regenerate the HPLC column.

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