Oligonucleotide Purification and Analysis: Method Development and Troubleshooting Strategies

(Course No. SS0-9373)

Date and Location
This course is split over 2 half-days. Registration cost covers both dates.

12 and 13 June 2024
08:30 - 12:30 BST | 09:30 - 13:30 CEST
Online

Price (per person)
£425 | 485 € | 480 CHF | 1200 PLN | 3600 DKK | 5100 NOK | 5350 SEK | 9300 CZK (+VAT, incl. PDF of the slides and certificate of attendance)

Course Summary

In this training we will discuss current chromatographic techniques being used as well as discuss some of the challenges people have encountered with their oligonucleotide LC and LC-MS separations.

Course Outline

Part 1: Oligonucleotides
  • A Primer
  • Analytical Challenges
Part 2: Applications
  • Mobile Phases
  • Column Phases
  • Sample Preparation
Part 3: Method Development
  • Flow Rate
  • Temperature
  • Method Optimizations
Part 4: Good Laboratory Practices

Practical Skills Acquired

As many organizations pivot towards developing Oligonucleotide Therapeutics, there is a widespread lack of knowledge in how to develop and optimize the various purification and analysis methods used to look at Oligonucleotide Therapeutics.
This course will provide you with the following practical knowledge:

  • Apply chromatography to oligo workflows
  • Fundamentals of LC and SPE for oligos
  • Mobile phase and column selection
  • Oligo applications and method optimization

Online Registration

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