Oligonucleotide Purification and Analysis

(Course No. SS0-9373)

Date and Location
On demand - Online

Please note: the seminar takes place on two half days (2 sessions of 4 hours each). Minimum of 4 attendees.
08:30 - 12:30 GMT | 09:30 - 13:30 CET

Price (per person)
£425 | 485 € | 480 CHF | 1200 PLN | 3600 DKK | 5100 NOK | 5350 SEK | 9300 CZK (+VAT, incl. PDF of the slides and certificate of attendance)

Course Summary

In this seminar we will discuss current chromatographic techniques being used as well as some of the challenges people have encountered with their oligonucleotide LC and LC-MS separations

Course Outline

Part 1: Oligonucleotides
  • A Primer
  • Analytical Challenges
Part 2: Applications
  • Mobile Phases
  • Column Phases
  • Sample Preparation
Part 3: Method Development
  • Flow Rate
  • Temperature
  • Column selection
Part 4: Good Laboratory Practices

Practical Skills Acquired

As many organisations pivot towards developing oligonucleotide therapeutics, there is a widespread lack of knowledge in how to develop and optimise the various purification and analysis methods used to look at oligonucleotide therapeutics.
This course will provide you with the following practical knowledge:

  • Apply chromatography to oligo workflows
  • Fundamentals of LC and SPE for oligos
  • Mobile phase and column selection
  • Oligo applications and method optimisation

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