Method Development for Liquid Chromatography

(Course No. SS0-9381)

Date and Location
17 April 2024
08:30 - 15:30 (UTC+2)
GLO Hotel Art
Lönnrotinkatu 29, 00180 Helsinki
Finland

19 April 2024
08:30 - 15:30 (CEST)
Karolinska Institute
Lärosal Ragnar Granit
Våningsplan 3 i Biomedicum
Solnavägen 9, 171 65 Solna
Sweden

Price (per person)
1195 SEK | 150 EUR (+VAT, incl. lunch and refreshments, seminar slides and certificate of attendance)

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Course Summary

In this seminar we provide an overview of the theory and interactions taking place in a silica-based column, primarily focusing on the reverse phase mode of separation. An introduction to the most common variables will allow us to dive deeper into the process of developing and improving methods for liquid chromatography with the ultimate goal of increased resolution.

Course Outline
  • Analytes and interactions - LogP, dissolution, pKa, interactions with stationary phase, and separation modes
  • Column dimensions - column ID, length, particle size, hardware, and limitations
  • Stationary phase - selectivity, interactions, and special phases (high pH, polymer, aqueous stable)
  • Mobile phase - selectivities of organics, buffer selection (pH), and gradient slope
  • Other parameters - flow rate, temperature, connections, injection programs, and sample diluent
Practical Skills Acquired

  • Minimize time spent selecting a phase and column dimension for an unknown analyte or new method.
  • Gain insight into how changes with the mobile phase can impact the overall resolution of a column and phase.
  • Understand how common LC parameters can be adjusted during the development of a method.
  • Improve existing method parameters to improve the separation of challenging analytes.
Presenter: Genevieve Hodson

Genevieve graduated from the University of Texas with a BS in Chemistry located in her hometown of Austin Texas. Out of school she spent 4 years as an Analytical Chemist in the QC and R&D Department of Cerilliant (a Sigma Millipore Company). There she worked with many analytical instruments but became an expert at LC by performing purity analysis, customer specific methods, validations, QC testing and stability studies on a wide range of small molecules including pharmaceuticals, pesticides, explosives and illegal substances. From there she moved to Los Angeles California where she worked at Johnson and Johnson and then a small dietary supplement company. After spending a year abroad, Genevieve joined the Phenomenex team first as a Technical Specialist in 2017, then as Manager of Technical Support, a global team who help Phenomenex customers every day with all their chromatography needs!

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