Date and Location
On site or Online - on demand
Course Summary
This training provides an in-depth overview of intact protein analysis for biopharmaceutical characterization. Learn top-down and middledown approaches for monoclonal antibodies, including reversed-phase strategies, LC particle selection, and method optimization. The course covers best practices for impurity profiling, glycoform identification, and post-translational modification analysis, offering practical tips for robust and reproducible workflows.
Course Outline
Introduction to Protein Analysis
- Overview of biopharmaceutical characterization
- Importance of intact protein workflows
Chromatographic Techniques
- RP, IEX, SEC, HIC, and Native LC-MS applications
Intact Protein and Glycan Characterization
- Top-down and middle-down approaches
- Glycan analysis and orthogonal techniques
Method Development and Practical Insights
- Column and mobile phase selection
- Optimization strategies and troubleshooting
- Real-world case studies and best practices
Practical Skills Acquired
This course will provide you with the following practical knowledge:
- Develop and optimize LC methods for intact protein analysis (RP, IEX, SEC, HIC, Native).
- Apply top-down and middle-down workflows for monoclonal antibody characterization.
- Perform sample preparation for intact proteins and glycan analysis
- Select appropriate stationary phases and mobile phases for different protein separations.
- Troubleshoot common issues in chromatographic separations of large biomolecules.
- Interpret chromatographic data for charge variants, aggregates, and glycoforms.
- Implement best practices for improving resolution, recovery, and reproducibility in biopharma workflows.
Have a question about these seminars? Please contact us
Nordic Office
DK: 4824 8048 | NO: +47 810 02 005SE: 08 611 6950 | FI: +358 (0)9 4789 0063
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