Chromatography Webinars

Live Chromatography Webinars 2024

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HPLC System Optimization

Date: 25 January 2024

Time: 10:00 - 11:00 (GMT) | 11:00 - 12:00 (CET)

Key Learning Points:

  • Learn how to optimize your HPLC system to enhance performance by minimizing extra column volume that can result in sample dispersion before the column and band broadening after the column.
  • Understand how to easily ensure zero-dead volume connections throughout your HPLC system.
  • Tips and Tricks for dispersion before and after the column can be reduced.
  • Who should attend:

    Anyone working with HPLC in the laboratory, but specifically those working in generic pharma quality control labs.

    Presenter: Phil Koerner, PhD, Pharmaceutical Market Development Manager, Phenomenex

    Effortless SPE Method Development in a Single Experiment

    Date: 14 May 2024

    Time: 10:00 - 11:00 (BST) | 11:00 - 12:00 (CEST)

    Key Learning Points:

  • Determine the best SPE sorbent and starting conditions for your analytes in a single run
  • Conserve valuable lab resources by spending less time developing SPE methods
  • Learn how to quickly identify SPE conditions for multi-analyte panels
  • Presenter: Stephanie J. Marin, Ph.D. Biography Global Market Development Manager - Clinical, Phenomenex

    Allowed Adjustments to Pharmacopeia HPLC Methods

    Date: 21 May 2024

    Time: 10:00 - 11:00 (BST) | 11:00 - 12:00 (CEST)

    Key Learning Points:

  • Review of recent revision to allowed adjustments for USP and Ph. Eur. HPLC methods
  • Utilize a case study to highlight the approach for making adjustments to an isocratic method and the resulting benefits
  • Understand the benefits of working within the allowed adjustments for improving the performance of compendial HPLC methods (isocratic and gradient) in your laboratory
  • Overview

  • Intro: Review revisions to allowed adjustments for USP and Ph. Eur.
  • Isocratic Case Study: Adjustments to column length and ID, particle size, and flow rate
  • Gradient Case Study: Adjustments to column length and ID, particle size, flow rate, and gradient timetable
  • Who should attend

    Scientists and analysts in the pharmaceutical industry who work with Pharmacopeia HPLC methods that are interested in improving performance, reducing analysis time, and understanding allowable adjustment application examples.

    Presenter: Dr. Phil Koerner, Global Pharmaceutical Market Development Manager, Phenomenex

    Identifying and Avoiding False Positives During Routine PFAS Analysis

    Date: 4 June 2024

    Time: 10:00 - 11:00 (BST) | 11:00 - 12:00 (CEST)


  • The reporting limit of a method is also dependent upon the consistency of clean blanks and elimination of sporadic contamination.
  • Vendors of PFAS consumables need to provide certified, trace level PFAS free products.
  • PFAS are everywhere! They are widely used, vendors/suppliers when contacted don't know their processes are using them.
  • Since PFAS contamination has become an issue with PFAS analysis all field samples are taken in duplicate, not just field blanks.
  • The complete data package and replicates need to be evaluated by the user in order to make sound conclusions and identify false positives.
  • Every step there is a chance to incorporate “free-range” PFAS in your sample, minimize the preparation steps.
  • How PFAS is analyzed at EPA Region 5 Laboratory.
  • Key Learning Points:

  • Learn how to approach analyzing for PFAS, it is not rocket science like they made it out to be.
  • Become aware of Sporadic PFAS Contamination.
  • Understand how to identify false positives.
  • Require all analysis quality control and samples to be analyzed in replicates to identify false positives.
  • Who should attend?

    Anyone lab scientists, or managers involved in PFAS Analysis.

    Presenters: Larry Zintek, Organic Methods Group Leader, UA EPA
    & Maryam Sayeed, Product Management Specialist, Phenomenex

    Evaluation of AAV Aggregation & Capsid Load (Empty / Full) CQAs

    Date: 6 June 2024

    Time: 10:00 - 11:00 (BST) | 11:00 - 12:00 (CEST)


    AAVs are attractive vehicles for targeted drug delivery. There are numerous Critical Quality Attributes (CQAs) evaluated to ensure safety and efficacy of AAVs. Aggregation is a CQA and should be controlled as AAVs are prone to aggregation from freeze thaw cycles during storage.

  • In this webinar, we will explore CQAs using a novel size exclusion column optimal for AAV analysis, which enables the separation of AAV monomer from aggregate species with excellent peak shape, resolution and peak profile to provide better data quality. Due to both the inert surface chemistry and flow-path in the BiozenTM dSEC-7 column, the analysis conditions were independent of the serotype showcasing the utilization of platform methodology.
  • In addition to calculating the aggregation level, aggregate molar mass, molar masses of protein and nucleic acid, and extinction coefficients of capsid and DNA were also determined. The novel particle chemistry of Biozen dSEC-7 provides instant stabilization of sensitive detectors like MALS to provide quality data.
  • Additionally, Full-to-Empty capsid ratio was determined to accurately predict capsid load demonstrating Biozen dSEC-7’s utility as a holistic solution for the determination of CQAs of AAVs.
  • Key Learning Points:

  • Learn how to optimize AAV aggregate analysis.
  • Understand how size exclusion can be effectively utilized for capsid load quantification.
  • Find out how optimized column specifications can improve data quality to determine critical quality attributes
  • Who should attend?

    Any scientist, associate or manager that optimizes AAV aggregate analysis methods.

    Presenter: Claire Butré, PhD, R&D Technical Leader, Quality Assistance
    & Ramkumar Dhandapani, PhD, Senior Product Management, Phenomenex

    Nitrosamines in Drug Products: Where Are We Now?

    Date: 13 June 2024

    Time: 10:00 - 10:40 (BST) | 11:00 - 11:40 (CEST)


    Since 2018 when the presence of Nitrosamines was first reported in several ARB (angiotensin II receptor blocker) drugs, there has been increasing regulatory focus to mitigate the presence of these potential human carcinogens in drug substances and drug products.

    Key Learning Points:

  • Review the latest regulatory updates (FDA and EMA).
  • Learn about published LC-MS/MS applications which address the N-Nitrosamines and NDSRI analysis challenges in drug products
  • Find solutions to sample preparation challenges for N-Nitrosamines analysis in drug products.
  • Obtain examples of specific Phenomenex applications for nitrosamines (10 mutagenic nitrosamine impurities in Acyclovir) and NDSRIs (N-nitroso propranolol in a propranolol drug substance and product).
  • Who should attend?

  • Anyone with an interest in, or challenged by, the analysis N-Nitrosamines and Nitrosamine Drug Substance Related Impurities (NDSRI) in API and/or Drug Products.
  • Analysts and Scientists working in the Pharmaceutical and Generic Pharmaceutical industries.
  • Presenter: Dr. Phil Koerner, Market Development Manager, Pharmaceuticals, Phenomenex

    Oligonucleotide Analysis in Tissue Samples

    Date: 20 June 2024

    Time: 10:00 - 10:00 (BST) | 11:00 - 12:00 (CEST)


  • Brief introduction to Oligonucleotides.
  • Sample Preparation Challenges.
  • Enrichment Workflow for Biological Fluids and Tissues.
  • Tips and Tricks for Best Results.
  • Key Learning Points:

  • Generalities on oligonucleotides and their role in gene therapy.
  • The major challenges of analyzing oligonucleotides in biological samples
  • Examples of sample preparation workflows focusing on biological fluids and tissues.
  • Workflow optimization to achieve the best results.
  • Who should attend?

    Any scientist new to working with oligonucleotides and scientists interested in improving their workflows for the enrichment and analysis of oligonucleotides from biological samples

    Presenter: Lucia Geis Asteggiante, Senior Technical Specialist, Phenomenex

    How to Take Care of Your LC Column

    Date: 29 August 2023

    Time: 10:00 - 11:00 (BST) | 11:00 - 12:00 (CEST)

    Key Learning Points:

  • Understand best practices upon opening a new LC Column.
  • Learn how to clean and regenerate an LC Column.
  • Find out about additional products and practices that can be used to minimize contamination getting onto LC columns.
  • Who should attend?

  • Any LC user who would like to ensure that their LC columns are properly cared for to make sure to get most life out of it that is possible.
  • Presenter: Genevieve Hodson, Technical Support, Phenomenex

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