Chromatography Webinars

Live Chromatography Webinars 2023

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5 Habits That Can Make Your LC Methods More Consistent

Date: 31. January 2023

Time: 10:00 - 11:00 (GMT) | 11:00 - 12:00 (CET)

Key Learning Points:

  • Understand the role of pH and the importance of buffers in mobile phase.
  • Lean the basics of installation and conditioning of the column
  • Find new tips to help with column equilibration and maintaining column temperature.
  • Presenter: Rajesh Babu Dandamudi, PhD, Technical Manager, Phenomenex

    How to Solvent Switch Successfully

    Date: 6 December 2023

    Time: 10:00 - 11:00 (GMT) | 11:00 - 12:00 (CET)

    Key Learning Points:

  • Gain a comprehensive understanding of what is solvent miscibility and why is it important to understand.
  • Why it is necessary to properly solvent switch a column and what can happen if it is not done correctly.
  • A general procedure to switch an LC column from one solvent to another.
  • Who should attend

    Any LC users looking to utilize different modes of separation and are required to switch a column that is shipped with an immiscible solvent to the one method requires

    Presenter: Genevieve Hodson, Technical Support, Phenomenex

    Critical Quality Attributes for Adeno-Associated Virus Analysis

    Date: 14 December 2023

    Time: 10:00 - 11:00 (GMT) | 11:00 - 12:00 (CET)

    Key Learning Points:

  • Explore modern chromatographic techniques for AAV quantification and characterization.
  • Gain a comprehensive understanding of Critical Quality Attributes (CQAs) and their significance in drug development.
  • Learn to navigate common CQA challenges at different development stages for meeting industry standards.
  • Discover the importance of selecting tailored chemistries and method conditions to ensure robust, efficient, and precise analytical methods.
  • Who should attend?

    Any Analytical Development Scientists, QC Analysts / Managers, or R&D Scientists who are looking to learn more about the role of chromatography in ensuring AAV quality and safety in drug development.

    Presenter: Shilin Lok Yee Cheung, Sr. Research Scientist, Phenomenex

    FDA’s Analytical Method for PFAS Detection

    Date: 21 November 2023

    Time: 10:00 - 11:00 (GMT) | 11:00 - 12:00 (CET)


    The development and expansion of analytical methods for per- and polyfluoroalkyl substances (PFAS) in food is essential for the continued monitoring of the United States (US) food supply and assessments of dietary intake. In March of 2022, the European Union Reference Laboratory (EURL) for halogenated persistent organic pollutants (POPs) in Feed and Food released a guidance document covering priority PFAS of interest, including analytical method parameters and limits of quantification (LOQs). As a result, the Food and Drug Administration (FDA) began method extension work to incorporate ten new additional analytes to method C-010.02 including long chain perfluorosulfonic acids, fluorotelomer sulfonates, and perfluorooctane sulfonamide. In December of 2022, The European Union published Commission Regulation 2022/2388, establishing maximum levels for perfluorooctane sulfonic acid (PFOS), perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), and perfluorohexane sulfonic acid (PFHxS) in certain foodstuffs, primarily fish, molluscs, crustaceans, and eggs. As a result, the FDA method was evaluated for performance in reaching EU required LOQs for both compliance and monitoring purposes. The FDA method was found to be able to reach all required LOQs for analytes in matrices with established maximum levels and with further method modifications, required LOQs could be met in fruits, vegetables, and milk.

    Key Learning Points:

  • Understand the FDA’s approach for PFAS LOQs in various food matrices.
  • Learn about the EU reference lab guidance for PFAS in foods and how the FDA is taking a similar approach to food safety.
  • Take a deep dive into which PFAS are designated targets and which are being monitored for screening .
  • Who should attend?

    Any scientists, lab analysts, food safety advocates, food manufacturers or anyone interested in food safety.

    Presenters: Susan Genualdi Ph.D. Research Chemist, Food and Drug Administration
    & Richard F. Jack Ph.D., Global Market Development Manager, Phenomenex

    Method Robustness and Transferability for Modern Size-Exclusion Methods

    Date: 23 November 2023

    Time: 10:00 - 11:00 (GMT) | 11:00 - 12:00 (CET)

    Key Learning Points:

  • Understand the impact of HPLC/UHPLC systems, including bio-inert and traditional stainless-steel configurations.
  • Learn how to transition from a legacy SEC method to improve analytical method throughput and data quality.
  • Determine the experimental parameters to vary to ensure demonstration of analytical method robustness .
  • Who should attend?

    Any scientists who are looking to learn more about method parameters and variables to consider for developing a robust and transferable method.

    Presenter: Helen Whitby, PhD, Senior Product Manager – Bioseparations, Phenomenex

    Spooktacular Solutions for LC Tubing and Connections

    Date: 31 October 2023

    Time: 10:00 - 11:00 (GMT) | 11:00 - 12:00 (CET)

    Key Learning Points:

  • Learn about types of tubing and fittings and making the best connections.
  • Make sense of how to select the correct tube, properly cutting it and choosing the right fitting.
  • Understanding extra-column volume and what are Zero-Dead Volume connections.
  • Tips and tricks to diagnose and troubleshoot poor tubing and/or fitting choices.
  • Who should attend?

    Any LC users wanting to deepen their knowledge of LC tubing and connections, including troubleshooting, to smoothly run their LC experiments.

    Presenter:Lucia Geis Asteggiante, Senior Technical Specialist, Phenomenex

    Case Studies: Allowed Adjustments to Pharmacopeia HPLC Methods

    Date: 28 September 2023

    Time: 10:00 - 10:00 (BST) | 11:00 - 12:00 (CEST)


    This webinar will provide a detailed understanding of new revisions to allowed adjustments for USP and Ph. Eur. HPLC methods through the use of case studies.

  • Intro: Review revisions to allowed adjustments for USP and Ph. Eur.
  • Isocratic Case Study: Adjustments to column length and ID, particle size, and flow rate.
  • Gradient Case Study: Adjustments to column length and ID, particle size, flow rate, and gradient timetable.
  • Key Learning Points:

  • Review of recent revision to allowed adjustments for USP and Ph. Eur. HPLC methods.
  • Utilize a case study to highlight the approach for making adjustments to an isocratic method and the resulting benefits.
  • Understand the benefits of working within the allowed adjustments for improving the performance of compendial HPLC methods (isocratic and gradient) in your laboratory.
  • Who should attend?

    Scientists and analysts in the pharmaceutical industry who work with Pharmacopeia HPLC methods that are interested in:
  • Improving performance.
  • Reducing analysis time.
  • Understanding allowable adjustment application examples.
  • Presenter: Dr. Phil Koerner, Global Pharmaceutical Market Development Manager, Phenomenex

    How to Take Care of Your LC Column

    Date: 29 August 2023

    Time: 10:00 - 11:00 (BST) | 11:00 - 12:00 (CEST)

    Key Learning Points:

  • Understand best practices upon opening a new LC Column.
  • Learn how to clean and regenerate an LC Column.
  • Find out about additional products and practices that can be used to minimize contamination getting onto LC columns.
  • Who should attend?

  • Any LC user who would like to ensure that their LC columns are properly cared for to make sure to get most life out of it that is possible.
  • Presenter: Genevieve Hodson, Technical Support, Phenomenex

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