Chromatography Webinars

Live Chromatography Webinars 2024

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HPLC System Optimization

Date: 25 January 2024

Time: 10:00 - 11:00 (GMT) | 11:00 - 12:00 (CET)

Key Learning Points:

  • Learn how to optimize your HPLC system to enhance performance by minimizing extra column volume that can result in sample dispersion before the column and band broadening after the column.
  • Understand how to easily ensure zero-dead volume connections throughout your HPLC system.
  • Tips and Tricks for dispersion before and after the column can be reduced.
  • Who should attend:

    Anyone working with HPLC in the laboratory, but specifically those working in generic pharma quality control labs.

    Presenter: Phil Koerner, PhD, Pharmaceutical Market Development Manager, Phenomenex

    How To Start Up and Shut Down LC and LC-MS Instruments

    Date: 31 January 2024

    Time: 10:00 - 11:00 (GMT) | 11:00 - 12:00 (CET)


  • Getting your system ready.
  • LC System Start Up and Shut Down.
  • LC-MS System Start Up and Shut Down.
  • On and Off System Column Storage.
  • Key Learning Points:

  • Best practices for getting your LC system prepared before you turn it on.
  • A general, system egnostic, approach to starting and stopping both a LC and LC-MS system.
  • How to store a column both on and off of a system as well as how to clean the column to best protect it from premature degradation.
  • Who should attend:

    Any user newer to Liquid Chromatography or using a Mass Spectrometer that would like to have a better understanding of protecting their column during system start up and shut down.

    Presenter: Genevieve Hodson, Technical Support, Phenomenex

    Optimizing PFAS Analysis for Draft Method 1633 Using a Stacked SPE Format

    Date: 29 February 2024

    Time: 10:00 - 11:00 (GMT) | 11:00 - 12:00 (CET)

    Key Learning Points:

  • Learn options for SPE phases for PFAS extraction from water and soil samples.
  • Utilize acceptable options for EPA 1633 as a guidance method.
  • Understand how to optimize time and cost for sample preparation.
  • Overview

    This webinar will present data and discuss EPA Draft Method 1633 compliant options to simplify sample clean up and increase sample throughput for PFAS analysis.

    Presenters: Philip Bassignani, Senior Director of Research and Development, Alpha Analytical & Richard Jack, Global Market Development Manager, Food and Environmental, Phenomenex

    FDA’s Analytical Method for PFAS Detection

    Date: 21 November 2023

    Time: 10:00 - 11:00 (GMT) | 11:00 - 12:00 (CET)


    The development and expansion of analytical methods for per- and polyfluoroalkyl substances (PFAS) in food is essential for the continued monitoring of the United States (US) food supply and assessments of dietary intake. In March of 2022, the European Union Reference Laboratory (EURL) for halogenated persistent organic pollutants (POPs) in Feed and Food released a guidance document covering priority PFAS of interest, including analytical method parameters and limits of quantification (LOQs). As a result, the Food and Drug Administration (FDA) began method extension work to incorporate ten new additional analytes to method C-010.02 including long chain perfluorosulfonic acids, fluorotelomer sulfonates, and perfluorooctane sulfonamide. In December of 2022, The European Union published Commission Regulation 2022/2388, establishing maximum levels for perfluorooctane sulfonic acid (PFOS), perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), and perfluorohexane sulfonic acid (PFHxS) in certain foodstuffs, primarily fish, molluscs, crustaceans, and eggs. As a result, the FDA method was evaluated for performance in reaching EU required LOQs for both compliance and monitoring purposes. The FDA method was found to be able to reach all required LOQs for analytes in matrices with established maximum levels and with further method modifications, required LOQs could be met in fruits, vegetables, and milk.

    Key Learning Points:

  • Understand the FDA’s approach for PFAS LOQs in various food matrices.
  • Learn about the EU reference lab guidance for PFAS in foods and how the FDA is taking a similar approach to food safety.
  • Take a deep dive into which PFAS are designated targets and which are being monitored for screening .
  • Who should attend?

    Any scientists, lab analysts, food safety advocates, food manufacturers or anyone interested in food safety.

    Presenters: Susan Genualdi Ph.D. Research Chemist, Food and Drug Administration
    & Richard F. Jack Ph.D., Global Market Development Manager, Phenomenex

    Method Robustness and Transferability for Modern Size-Exclusion Methods

    Date: 23 November 2023

    Time: 10:00 - 11:00 (GMT) | 11:00 - 12:00 (CET)

    Key Learning Points:

  • Understand the impact of HPLC/UHPLC systems, including bio-inert and traditional stainless-steel configurations.
  • Learn how to transition from a legacy SEC method to improve analytical method throughput and data quality.
  • Determine the experimental parameters to vary to ensure demonstration of analytical method robustness .
  • Who should attend?

    Any scientists who are looking to learn more about method parameters and variables to consider for developing a robust and transferable method.

    Presenter: Helen Whitby, PhD, Senior Product Manager – Bioseparations, Phenomenex

    Spooktacular Solutions for LC Tubing and Connections

    Date: 31 October 2023

    Time: 10:00 - 11:00 (GMT) | 11:00 - 12:00 (CET)

    Key Learning Points:

  • Learn about types of tubing and fittings and making the best connections.
  • Make sense of how to select the correct tube, properly cutting it and choosing the right fitting.
  • Understanding extra-column volume and what are Zero-Dead Volume connections.
  • Tips and tricks to diagnose and troubleshoot poor tubing and/or fitting choices.
  • Who should attend?

    Any LC users wanting to deepen their knowledge of LC tubing and connections, including troubleshooting, to smoothly run their LC experiments.

    Presenter:Lucia Geis Asteggiante, Senior Technical Specialist, Phenomenex

    Case Studies: Allowed Adjustments to Pharmacopeia HPLC Methods

    Date: 28 September 2023

    Time: 10:00 - 10:00 (BST) | 11:00 - 12:00 (CEST)


    This webinar will provide a detailed understanding of new revisions to allowed adjustments for USP and Ph. Eur. HPLC methods through the use of case studies.

  • Intro: Review revisions to allowed adjustments for USP and Ph. Eur.
  • Isocratic Case Study: Adjustments to column length and ID, particle size, and flow rate.
  • Gradient Case Study: Adjustments to column length and ID, particle size, flow rate, and gradient timetable.
  • Key Learning Points:

  • Review of recent revision to allowed adjustments for USP and Ph. Eur. HPLC methods.
  • Utilize a case study to highlight the approach for making adjustments to an isocratic method and the resulting benefits.
  • Understand the benefits of working within the allowed adjustments for improving the performance of compendial HPLC methods (isocratic and gradient) in your laboratory.
  • Who should attend?

    Scientists and analysts in the pharmaceutical industry who work with Pharmacopeia HPLC methods that are interested in:
  • Improving performance.
  • Reducing analysis time.
  • Understanding allowable adjustment application examples.
  • Presenter: Dr. Phil Koerner, Global Pharmaceutical Market Development Manager, Phenomenex

    How to Take Care of Your LC Column

    Date: 29 August 2023

    Time: 10:00 - 11:00 (BST) | 11:00 - 12:00 (CEST)

    Key Learning Points:

  • Understand best practices upon opening a new LC Column.
  • Learn how to clean and regenerate an LC Column.
  • Find out about additional products and practices that can be used to minimize contamination getting onto LC columns.
  • Who should attend?

  • Any LC user who would like to ensure that their LC columns are properly cared for to make sure to get most life out of it that is possible.
  • Presenter: Genevieve Hodson, Technical Support, Phenomenex

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