Chromatography Webinars

Live Chromatography Webinars 2023

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New Sample Prep Tips and Tricks for the Extraction of Polar Analytes

Date: 25. January 2022

Time: 10:00 - 11:00 (GMT) | 11:00 - 12:00 (CET)

Key Learning Points:

  • Identify the most common challenges analyst face when working with polar compounds and how to sort them out.
  • Walk through the method of a proven and effective sample prep technique to extract polar analyte from various sample matrices successfully.
  • Examine data analysis of multiple studies and applications showing comparisons of reproducibility, sample recovery, and cost efficiency.
  • Presenter: Ramkumar Dhandapani, PhD, Global Product Manager Gas Chromatography, Phenomenex

    5 Habits That Can Make Your LC Methods More Consistent

    Date: 31. January 2023

    Time: 10:00 - 11:00 (GMT) | 11:00 - 12:00 (CET)

    Key Learning Points:

  • Understand the role of pH and the importance of buffers in mobile phase.
  • Lean the basics of installation and conditioning of the column
  • Find new tips to help with column equilibration and maintaining column temperature.
  • Presenter: Rajesh Babu Dandamudi, PhD, Technical Manager, Phenomenex

    Optimizing PFAS Analysis via Draft Method 1633 Using a Stacked SPE Format

    Date: 25. May 2023

    Time: 10:00 - 11:00 (BST) | 11:00 - 12:00 (CEST)

    Key Learning Points:

  • Learn options for SPE phases for PFAS extraction from water and soil samples.
  • Utilize acceptable options for EPA 1633 as a guidance method.
  • Understand how to optimize time and cost for sample preparation.
  • Event Overview

  • This webinar will present data and discuss EPA Draft Method 1633 compliant options to simplify sample clean up and increase sample throughput for PFAS analysis.
  • Presenters: Presenters: Richard Jack, Global Market Development Manager, Food and Environmental, Phenomenex & Philip Bassignani, Senior Director of Research and Development, Alpha Analytical

    SEC-nHRMS for the Characterization of mAbs

    Date: 15. February 2023

    Time: 10:00 - 11:00 (GMT) | 11:00 - 12:00 (CET)

    Key Learning Points:

  • Receive a demonstration of how non-denaturing SEC-Native MS can be leveraged to streamline mAb characterization, including methods for the digestion of mAbs.
  • Listen in to a column selection discussion, which includes pore volume and column dimensions to ensure monomer and aggregate resolution, and optimize mobile phases for complex characterizations.
  • Learn about SEC combined with nHRMS, and how to select a SEC column.
  • Who should attend?

  • Any scientists wanting to learn how to combine their size exclusion chromatography and High-resolution Mass Spec for improvements in their mAb characertizations.
  • Presenter: Lorne Nelson Senior Scientist - Field Applications, Phenomenex

    HPLC Toubleshooting

    Date: 15. June 2023

    Time: 10:00 - 11:00 (BST) | 11:00 - 12:00 (CEST)

    Key Learning Points:

  • Learn how to prevent and minimize problems before they occur.
  • Understand how to approach problem solving and troubleshooting in a logical way, including: diagnose cause and possible solutions.
  • Review peak shape, and column performance problems.
  • Who should attend?

  • Any LC users who would like to get tips on extend the column lifetime.
  • Presenter: Kevin Zhao, Senior Technical Specialist, Phenomenex

    Chiral Chromatography Screening 101

    Date: 22. June 2023

    Time: 10:15 - 11:15 (BST) | 11:15 - 12:15 (CEST)

    Key Learning Points:

  • Learn why chiral screening is done, what are the benefits.
  • Take a deep dive into columns, and screening modes/methods.
  • Listen into sample compatibility considerations including: detection, solubility, and chiral centers.
  • Understand quick, easy, and cost-effective ways to identify a solution for your chiral separation needs.
  • Who should attend?

  • Any LC users who would like to get tips on a chiral screening process for method development.
  • Any academia or pharmaceutical scientists who need to rapidly develop a method for separation and characterization of their chiral compounds.
  • Presenter: Zandra Baja, Application Scientist, Phenomenex

    Practical Approaches for Identifying Equivalent and Orthogonal Columns

    Date: 27. June 2023

    Time: 10:00 - 11:00 (BST) | 11:00 - 12:00 (CEST)

    Key Learning Points:

    The selection of a suitable column for HPLC and UHPLC method development can be challenging. There is an immense number of columns to choose from, with almost 400 containing the ever-popular C18 phase alone and an ever-expanding offering of non-C18 stationary phases being introduced and marketed for everything from improved retention for polar analytes to improved peak shape for basic analytes. Columns that offer very similar selectivity can provide an alternative, or back up column, for a method. Columns having different selectivities may be desired when alternative, or orthogonal, separations are necessary, or when different elution orders are beneficial for complex separations. In this lecture we will review several different strategies for classifying and identifying similar and very different columns.

  • Identifying columns with similar selectivity to your current column of choice.
  • Identifying columns with different or orthogonal selectivity to your current column of choice.
  • Understanding the resources that are available for selectivity data for HPLC and UHPLC columns.
  • Who should attend?

  • Any LC users, in particular those who are working on SM Pharma and Generic Pharma.
  • Presenter: Heiko Behr, Senior Business Development Manager, Phenomenex

    PFAS EPA Method Development and Findings for UCMR5

    Date: 29. June 2023

    Time: 10:00 - 10:00 (BST) | 11:00 - 12:00 (CEST)


    The U.S. EPA, Office of Ground Water and Drinking Water, Technical Support Branch develops drinking water analytical methods supporting programs, such as the Unregulated Contaminant Monitoring Rule (UCMR) Program. The UCMR Program is a national occurrence study used to collect data for contaminants that are suspected to be present in drinking water and do not have health-based standards set under the Safe Drinking Water Act (SDWA). Rugged analytical methods are needed to quantify these contaminants, meaning the methods must yield reliable, accurate results over the range of field and lab conditions specified in the method. To ensure that analytical methods meet these criteria, EPA develops drinking water methods using a rigorous validation process that assesses the method performance under various conditions that would support method use at a national level. This validation process includes interference and contamination assessments, adherence to quality control acceptance criteria, quantitation limit determinations, sample preservation efficacies, and multi-laboratory comparisons. Per- and polyfluoroalkyl substances (PFAS) constitute a significant portion of the fifth UCMR cycle (UCMR 5). In this webinar, William Adams, PhD (EPA) will give an overview of EPA analytical method development and validation processes for drinking water methods supporting the UCMR Program, using EPA published and in-development PFAS analytical methods as examples. Agustin Pierri (Weck Laboratories) will add the experiences of a commercial laboratory doing PFAS analysis both in support of UCMR5 and for routine drinking water compliance monitoring. Weck Laboratories has been analyzing environmental samples for PFAS for over fifteen years and has developed a comprehensive understanding of the challenges associated with this type of analysis. In the first few months of UCMR5 testing and the first years of routine compliance monitoring in California, the lab has seen some interesting results. Dr. Pierri will cover the differences between the commonly used analytical methods and their applications, some of the challenges that may arise with each method, as well as the results and what they could mean for water quality. Suggestions on how to overcome these challenges and how to successfully deploy these methods in a commercial laboratory will also be examined.

    Key Learning Points:

  • Learn how the EPA Office of Ground Water and Drinking water optimizes and validates methods for UCMR5.
  • Understand how one lab is applying this method to determine PFAS in real water samples.
  • Find out what LODs and LOQs are seeing in the first six months of UCMR5.
  • Who should attend?

  • Anyone interested in PFAS contamination in drinking water and looking to sort out analytical challenges. .
  • Presenters: William A. Adams, Chemist, U.S. EPA & Agustin Pierri, Laboratory Technical Director, Weck Labs

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