Chromatography Webinars

Live Chromatography Webinars 2023

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New Sample Prep Tips and Tricks for the Extraction of Polar Analytes

Date: 25. January 2022

Time: 10:00 - 11:00 (GMT) | 11:00 - 12:00 (CET)

Key Learning Points:

  • Identify the most common challenges analyst face when working with polar compounds and how to sort them out.
  • Walk through the method of a proven and effective sample prep technique to extract polar analyte from various sample matrices successfully.
  • Examine data analysis of multiple studies and applications showing comparisons of reproducibility, sample recovery, and cost efficiency.
  • Presenter: Ramkumar Dhandapani, PhD, Global Product Manager Gas Chromatography, Phenomenex

    5 Habits That Can Make Your LC Methods More Consistent

    Date: 31. January 2023

    Time: 10:00 - 11:00 (GMT) | 11:00 - 12:00 (CET)

    Key Learning Points:

  • Understand the role of pH and the importance of buffers in mobile phase.
  • Lean the basics of installation and conditioning of the column
  • Find new tips to help with column equilibration and maintaining column temperature.
  • Presenter: Rajesh Babu Dandamudi, PhD, Technical Manager, Phenomenex

    Optimizing PFAS Analysis via Draft Method 1633 Using a Stacked SPE Format

    Date: 25. May 2023

    Time: 10:00 - 11:00 (BST) | 11:00 - 12:00 (CEST)

    Key Learning Points:

  • Learn options for SPE phases for PFAS extraction from water and soil samples.
  • Utilize acceptable options for EPA 1633 as a guidance method.
  • Understand how to optimize time and cost for sample preparation.
  • Event Overview

  • This webinar will present data and discuss EPA Draft Method 1633 compliant options to simplify sample clean up and increase sample throughput for PFAS analysis.
  • Presenters: Presenters: Richard Jack, Global Market Development Manager, Food and Environmental, Phenomenex & Philip Bassignani, Senior Director of Research and Development, Alpha Analytical

    LC Pressure 101

    Date: 19 September 2023

    Time: 10:00 - 11:00 (BST) | 11:00 - 12:00 (CEST)

    Key Learning Points:

  • Gain a comprehensive understanding of solvent miscibility and recognize its significance
  • Develop an in-depth comprehension of how to properly solvent switch a column, and the potential consequences associated with incorrect procedures
  • Familiarize yourself with a standardized protocol for transitioning an LC column from one solvent system to another, ensuring a seamless solvent switch
  • Who should attend?

  • Any LC users who are looking to understand the factors that can impact a systems backpressure and how to mitigate potential backpressure problems.
  • Presenter: Koen Askamp Technical Specialist, Phenomenex

    Fast and Robust Analysis of Anionic and Cationic Pesticides on a Single Column

    Date: 20 September 2023

    Time: 10:00 - 11:00 (BST) | 11:00 - 12:00 (CEST)

    Key Learning Points:

  • Overcome critical challenges in polar pesticides analysis and throughput.
  • Develop and optimize a robust method for cationic and anionic pesticides.
  • Learn new advances in column selectivity and MS for optimized separation of polar pesticides.
  • Who should attend?

  • Any LC users who want to learn about a faster yet robust analysis of polar pesticides or any lab managers who would need consolidation of anionic and cationic pesticides on a single column.
  • Presenter: Zara Jalali, Applications Scientist, Phenomenex

    HPLC Method Development: Practical Approaches for Identifying Equivalent and Orthogonal Columns

    Date: 21 September 2023

    Time: 10:00 - 11:00 (BST) | 11:00 - 12:00 (CEST)

    Reliable analytical method development and optimization for characterizating biotherapeutics is critical for assessing product quality and ensuring patient safety. Therefore, it is imperative to identify parameters that cause method variabilities and establish control strategies to ensure robustness and high data quality.
    In this interactive webinar part 1 of 2 webinar series, we will provide an overview of emerging particle technologies for the characterization and quality control of biotherapeutics as well as tips and tricks to ensuring robust method development and optimization.

    Key Learning Points:

  • Understand the methodologies and approaches of developing characterization methods for biotherapeutics.
  • Identify key method parameters that effect method robustness.
  • Establish control strategies that ensures robust performance and high quality data.
  • Who should attend?

  • Any method development scientists or anyone involved in optimizing/modernizing methods.
  • Presenter: Namrata Saxena, Technical Manager, Phenomenex

    Understanding Reverse Phase Selectivity for Different Compound Classes

    Date: 27 September 2023

    Time: 10:00 - 11:00 (BST) | 11:00 - 12:00 (CEST)


    The selection of a suitable column for HPLC and UHPLC method development can be challenging. There is an immense number of columns to choose from, with almost 400 containing the ever-popular C18 phase alone and an ever-expanding offering of non-C18 stationary phases being introduced and marketed for everything from improved retention for polar analytes to improved peak shape for basic analytes. Columns that offer very similar selectivity can provide an alternative, or back up column, for a method. Columns having different selectivities may be desired when alternative, or orthogonal, separations are necessary, or when different elution orders are beneficial for complex separations. In this lecture we will review several different strategies for classifying and identifying similar and very different columns.

    Key Learning Points:

  • Identifying columns with similar selectivity to your current column of choice.
  • Identifying columns with different or orthogonal selectivity to your current column of choice.
  • Understanding the resources that are available for selectivity data for HPLC and UHPLC columns.
  • Who should attend?

    Any LC users who are looking for are looking for a basic overview of Reverse Phase Method Development by looking at different small molecule compound classes

    Presenter: Genevieve Hodson, Senior Application Scientist, Phenomenex

    Case Studies: Allowed Adjustments to Pharmacopeia HPLC Methods

    Date: 28 September 2023

    Time: 10:00 - 10:00 (BST) | 11:00 - 12:00 (CEST)


    This webinar will provide a detailed understanding of new revisions to allowed adjustments for USP and Ph. Eur. HPLC methods through the use of case studies.

  • Intro: Review revisions to allowed adjustments for USP and Ph. Eur.
  • Isocratic Case Study: Adjustments to column length and ID, particle size, and flow rate.
  • Gradient Case Study: Adjustments to column length and ID, particle size, flow rate, and gradient timetable.
  • Key Learning Points:

  • Review of recent revision to allowed adjustments for USP and Ph. Eur. HPLC methods.
  • Utilize a case study to highlight the approach for making adjustments to an isocratic method and the resulting benefits.
  • Understand the benefits of working within the allowed adjustments for improving the performance of compendial HPLC methods (isocratic and gradient) in your laboratory.
  • Who should attend?

    Scientists and analysts in the pharmaceutical industry who work with Pharmacopeia HPLC methods that are interested in:
  • Improving performance.
  • Reducing analysis time.
  • Understanding allowable adjustment application examples.
  • Presenter: Dr. Phil Koerner, Global Pharmaceutical Market Development Manager, Phenomenex

    How to Take Care of Your LC Column

    Date: 29 August 2023

    Time: 10:00 - 11:00 (BST) | 11:00 - 12:00 (CEST)

    Key Learning Points:

  • Understand best practices upon opening a new LC Column.
  • Learn how to clean and regenerate an LC Column.
  • Find out about additional products and practices that can be used to minimize contamination getting onto LC columns.
  • Who should attend?

  • Any LC user who would like to ensure that their LC columns are properly cared for to make sure to get most life out of it that is possible.
  • Presenter: Genevieve Hodson, Technical Support, Phenomenex

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