Live Chromatography Webinars 2023

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New Sample Prep Tips and Tricks for the Extraction of Polar Analytes

Date: 25. January 2022

Time: 10:00 - 11:00 (GMT) | 11:00 - 12:00 (CET)

Key Learning Points:

  • Identify the most common challenges analyst face when working with polar compounds and how to sort them out.
  • Walk through the method of a proven and effective sample prep technique to extract polar analyte from various sample matrices successfully.
  • Examine data analysis of multiple studies and applications showing comparisons of reproducibility, sample recovery, and cost efficiency.

    • Presenter: Ramkumar Dhandapani, PhD, Global Product Manager Gas Chromatography, Phenomenex

    5 Habits That Can Make Your LC Methods More Consistent

    Date: 31. January 2023

    Time: 10:00 - 11:00 (GMT) | 11:00 - 12:00 (CET)

    Key Learning Points:

  • Understand the role of pH and the importance of buffers in mobile phase.
  • Lean the basics of installation and conditioning of the column
  • Find new tips to help with column equilibration and maintaining column temperature.

    • Presenter: Rajesh Babu Dandamudi, PhD, Technical Manager, Phenomenex

    SEC-nHRMS for the Characterization of mAbs

    Date: 15. February 2023

    Time: 10:00 - 11:00 (GMT) | 11:00 - 12:00 (CET)

    Key Learning Points:

  • Receive a demonstration of how non-denaturing SEC-Native MS can be leveraged to streamline mAb characterization, including methods for the digestion of mAbs.
  • Listen in to a column selection discussion, which includes pore volume and column dimensions to ensure monomer and aggregate resolution, and optimize mobile phases for complex characterizations.
  • Learn about SEC combined with nHRMS, and how to select a SEC column.

    • Who should attend?

  • Any scientists wanting to learn how to combine their size exclusion chromatography and High-resolution Mass Spec for improvements in their mAb characertizations.

    • Presenter: Lorne Nelson Senior Scientist - Field Applications, Phenomenex

    Novel Oligonucleotide Sample Clean-up and Method Optimizations

    Date: 21. February 2023

    Time: 10:00 - 10:00 (GMT) | 11:00 - 11:00 (CET)

    Key Learning Points:

  • An overview of sample preparation strategies for synthetic oligonucleotides, including how to adjust experimental design depending on the oligo and sample matrix.
  • Find ways ensure effective sample preparation and cleanup methods are implemented.
  • Learn about method optimization for small volume sample extraction and clean-up solutions for oligonucleotides will be explored to further optimize challenging methods.

    • Who should attend?

  • Any Bioanalytical chemists new to working with oligonucleotides, or anyone new to oligonucleotide characterization by LC-MS.

    • Presenter: Mihir Thakar, Global Product Manager, Phenomenex

    Maximizing Low Flow Separations with Core Shell Particle Technology

    Date: 28. February 2023

    Time: 10:00 - 10:00 (GMT) | 11:00 - 11:00 (CET)

    Summary:
    In this webinar we will learn about the use of core shell particle technology in Low Flow (micro- and nano-LC) applications. We will review micro- and nano-LC fundamentals and present a selective approach to trap-and-elute column strategies to increase method performance while providing examples of low flow applications using different column selectivities. In particular, the use of microflow LC-MS/MS to obtain sequence coverage and PTM information of Adeno-Associated Viruses (AVV) viral proteins will be highlighted. The benefits of using microflow for AAV proteins identification in comparison to analytical flow will be addressed.

    Key Learning Points:

  • Learn how to optimize the use of core shell particle technology and selectivities to improve low flow chromatographic separations.
  • Learn about the advantage of using micro-LC MS/MS for peptide mapping to provide sequence coverage and PTM information of AAV proteins.
  • Understand trap-and-elute – forward and backward comparisons.
  • Discover trap and column selectivity strategies to improve LC-MS/MS data quality when working with low flow applications.

    • Who should attend?

  • Scientists who are interested in improving and expanding their micro and nano-LC performance and would like to increase their knowledge of current trap and column strategies.

    • Presenter: Sergio Guazzotti, Ph.D., Business Development Manager, Micro/ Nano LC, Europe

    How to Pick a Phenyl Phase

    Date: 18. April 2023

    Time: 10:00 - 11:30 (BST) | 11:00 - 11:30 (CEST)

    Key Learning Points:

  • Learn about selectivity benefits of phenyl phases during reversed-phase HPLC.
  • Review mobile-phase optimization when using a phenyl column.
  • Examine a thorough comparison of different phenyl phases with a C18 phase.

    • Who should attend?

  • Any HPLC scientists beginning with method development or working with small molecules in pharmaceutical labs.

    • Presenter: Zachary Woodward, Senior Technical Specialist, Phenomenex

    HPLC column maintenance

    Date: 20. April 2023

    Time: 10:00 - 11:00 (BST) | 11:00 - 12:00 (CEST)

    Key Learning Points:

  • Learn what to do when you first receive your new column.
  • Find new tips and tricks to increase column lifetime.
  • Understand when it is time to say goodbye to your column.

    • Who should attend?

  • Any analysts or scientists routinely working with HPLC columns.

    • Presenter: Swetha Kotikalapudi, Technical Manager, Phenomenex

    How to Prepare a Mobile Phase

    Date: 27. April 2023

    Time: 10:00 - 11:00 (BST) | 11:00 - 12:00 (CEST)

    Key Learning Points:

  • Learn about mobile phases used in different separation modes .
  • Understand how to prepare a mobile phase that can deliver reproducible results, and mobile phase related troubleshooting.
  • Review the importance of degassing the mobile phase, and preparation of mobile phase for reversed phase chromatography.

    • Who should attend?

  • Anyone working with HPLC in the laboratory, or anyone struggling with reproducibility.

    • Presenter: Grace Guo, Senior Technical Specialist, Phenomenex

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