Chromatography Webinars EMEA | Phenomenex

Live Chromatography Webinars 2025

Discover ALL our upcoming webinars below

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HPLC System Optimization

Date: 25 January 2024

Time: 10:00 - 11:00 (GMT) | 11:00 - 12:00 (CET)

Key Learning Points:

  • Learn how to optimize your HPLC system to enhance performance by minimizing extra column volume that can result in sample dispersion before the column and band broadening after the column.
  • Understand how to easily ensure zero-dead volume connections throughout your HPLC system.
  • Tips and Tricks for dispersion before and after the column can be reduced.
  • Who should attend:

    Anyone working with HPLC in the laboratory, but specifically those working in generic pharma quality control labs.

    Presenter: Phil Koerner, PhD, Pharmaceutical Market Development Manager, Phenomenex

    Effortless SPE Method Development in a Single Experiment

    Date: 14 May 2024

    Time: 10:00 - 11:00 (BST) | 11:00 - 12:00 (CEST)

    Key Learning Points:

  • Determine the best SPE sorbent and starting conditions for your analytes in a single run
  • Conserve valuable lab resources by spending less time developing SPE methods
  • Learn how to quickly identify SPE conditions for multi-analyte panels
  • Presenter: Stephanie J. Marin, Ph.D. Biography Global Market Development Manager - Clinical, Phenomenex

    Allowed Adjustments to Pharmacopeia HPLC Methods

    Date: 21 May 2024

    Time: 10:00 - 11:00 (BST) | 11:00 - 12:00 (CEST)

    Key Learning Points:

  • Review of recent revision to allowed adjustments for USP and Ph. Eur. HPLC methods
  • Utilize a case study to highlight the approach for making adjustments to an isocratic method and the resulting benefits
  • Understand the benefits of working within the allowed adjustments for improving the performance of compendial HPLC methods (isocratic and gradient) in your laboratory
  • Overview

  • Intro: Review revisions to allowed adjustments for USP and Ph. Eur.
  • Isocratic Case Study: Adjustments to column length and ID, particle size, and flow rate
  • Gradient Case Study: Adjustments to column length and ID, particle size, flow rate, and gradient timetable
  • Who should attend

    Scientists and analysts in the pharmaceutical industry who work with Pharmacopeia HPLC methods that are interested in improving performance, reducing analysis time, and understanding allowable adjustment application examples.

    Presenter: Dr. Phil Koerner, Global Pharmaceutical Market Development Manager, Phenomenex


    Oligonucleotide Analysis in Tissue Samples

    Date: 20 June 2024

    Time: 10:00 - 10:00 (BST) | 11:00 - 12:00 (CEST)

    Overview:

  • Brief introduction to Oligonucleotides.
  • Sample Preparation Challenges.
  • Enrichment Workflow for Biological Fluids and Tissues.
  • Tips and Tricks for Best Results.
  • Key Learning Points:

  • Generalities on oligonucleotides and their role in gene therapy.
  • The major challenges of analyzing oligonucleotides in biological samples
  • Examples of sample preparation workflows focusing on biological fluids and tissues.
  • Workflow optimization to achieve the best results.
  • Who should attend?

    Any scientist new to working with oligonucleotides and scientists interested in improving their workflows for the enrichment and analysis of oligonucleotides from biological samples

    Presenter: Lucia Geis Asteggiante, Senior Technical Specialist, Phenomenex


    Optimizing PFAS Analysis for Method 1633 Using a Stacked SPE Format

    Date: 6 February 2025

    Time: 10:00 GMT | 11:00 CET

    Overview:

    This webinar will present data and discuss EPA Draft Method 1633 compliant options to simplify sample clean up and increase sample throughput for PFAS analysis.

    Key Learning Points:

  • Learn options for SPE phases for PFAS extraction from water and soil samples.
  • Utilize acceptable options for EPA 1633 as a guidance method.
  • Understand how to optimize time and cost for sample preparation.
  • Who should attend?

    Any scientists who wants to learn about solid phase extraction and it’s associated challenges, common troubleshooting steps and solutions to develop robust and reproducible extraction methods for their sample preparation needs.

    Presenters: Philip Bassignani Senior Director of Research and Development, Alpha Analytical
    & Richard Jack, Global Market Development Manager, Food and Environmental, Phenomenex


    Overview of Biomolecule Analysis

    Date: 13 February 2025

    Time: 10:00 - 11:00 (GMT) | 11:00 - 12:00 (CET)

    Overview:

    • Peptide therapeutics share familiar HPLC techniques
    • Oligonucleotides rely upon ion-pairing during HPLC
    • Large proteins and antibodies draw from a range of chromatographic techniques

    Key Learning:

    • Unravel biomolecule therapeutics from traditional small-molecule pharmaceuticals
    • Distinguish “biomolecules” from traditional small-molecule pharmaceuticals
    • Introduce the chromatographic techniques associated with different biomolecules

    Who should attend?

    Chemists who work primarily with small-molecule pharma drugs will be introduced to the variety of biomelcules that comprise the biopharma portfolio, along with the corresponding analytical techniques for HPLC.

    Presenter: Zachary Woodward, Senior Technical Specialist, Phenomenex


    Mastering SEC Biotherapeutic Characterization & Method Development

    Date: 25 March 2025

    Time: 10:00 - 11:00 (GMT) | 11:00 - 12:00 (CET)

    Overview:

    • The importance of SEC in biotherapeutic characterization, its mechanism of action, and theoretical principles.
    • Applications of SEC for proteins, antibodies, and other biologics, including method considerations for different sample types.
    • Key factors in selecting the correct LC column, buffer, pH, ionic strength, and flow rate to optimize separation and maintain sample integrity.
    • Characteristics of an effective and reproducible SEC method, ensuring accurate and consistent results.
    • Example workflows and recommended detector settings for specific biologics, including mAbs, AAV, mRNA, and sgRNA.

    Key Leaning Points:

    • Gain a deeper understanding of SEC theory, mechanism of action, and its role in biotherapeutic analysis.
    • Learn how to select optimal LC columns, buffers, and method conditions for different sample types.
    • Explore key considerations such as formulation stability, vial type, pH, and ionic strength for robust method development.
    • Identify the desirable attributes of a reproducible SEC method for improved analytical performance.
    • Review real-world SEC applications with detector settings optimized for mAbs, AAV, mRNA, and sgRNA characterization.

    Who should attend?

    • Researchers and Scientists: Ideal for those involved in the development and characterization of biotherapeutics.
    • Quality Control and In-Process Testing Professionals: Recommended for professionals focused on ensuring the quality and stability of biotherapeutic products.
    • Biopharmaceutical Industry Professionals: Valuable for those optimizing their SEC methods for biotherapeutic characterization.

    Presenter: Shilin Lok Yee Cheung Ph.D, Staff Scientist - New Product Development, Phenomenex


    Simultaneous Quantitation of Ultrashort-, Short-, and Long-Chain PFAS in Water by LC-MS/MS

    Date: 27 March 2025

    Time: 10:00 (GMT) | 11:00 (CET)

    Overview:

    This webinar introduces a direct injection LC-MS/MS method for the simultaneous quantitation of ultrashort-, short- and long-chain perfluoroalkyl acids (PFAAs) and other per- and polyfluoroalkyl substances (PFAS) in water. The use of mixed-mode chromatography in both the analytical and delay columns demonstrated robust retention and separation for the wide range of PFAS chain lengths tested. The sensitivity of the SCIEX 7500 system enabled in-sample limits of quantitation (LOQs) of 0.2–10 ng/L for most of the target analytes with good accuracy (±25%) and precision ( > 25%) performance. Trifluoroacetic acid (TFA), perfluorobutanoic acid (PFBA), 5:3 and 7:3 fluorotelomer carboxylic acids (FTCAs) and 8:2 polyfluoroalkyl phosphate diester (diPAP) required higher LOQs (10–100 ng/L) due to background contamination. Application of the method to tap water, rainwater and lake water revealed the detection of ultrashort-, short- and to a lesser extent, long-chain PFAAs at sub-ng/L to µg/L level.

    Key Learning Points:

  • Learn about using reversed PFAS chromatography from C1-C16 chain lengths.
  • Gain insights into the detection levels of ultrashort-, short-, and long-chain PFAAs, ranging from sub-ng/L to µg/L.
  • Understand the challenges posed by background contamination and delay column options required for short and long chain contaminants.
  • Who should attend?

    Any lab scientists and managers involved in PFAS Analysis.

    Guest Speaker: Holly Lee Food LCMS Scientist, Sciex


    Exploring Biomolecules: Oligonucleotide Basics Unleashed!

    Date: 10 April 2025

    Time: 10:00 - 10:40 (GMT) | 11:00 - 11:40 (CET)

    Overview:

    Oligonucleotide therapeutics are a widely accepted form of therapy because of their ability to treat diseases at the genetic level. Current advances in oligonucleotide therapeutic research push the boundaries of analytics to ensure safety and efficacy.

    Key Learning Points:

    • Learn about the basic building blocks and structures of oligonucleotides
    • Understand oligonucleotide modifications and how they impact the chemical and physical properties
    • Explore how oligonucleotides function as a biotherapeutic
    • Expand knowledge of analyzing oligonucleotides and address current analytical challenges

    Who should attend?

    This webinar is tailored for scientists, researchers, and industry professionals working in oligonucleotide development, manufacturing, and analytical testing that wish to learn more about oligonucleotides and its applications.

    Presenter: Namrata Saxena, Technical Manager, Phenomenex


    Eliminating Matrix Effects in Lipid-rich, Full-Fish Homogenates

    Date: 15 April 2025

    Time: 10:00 - 11:00 (GMT) | 11:00 - 12:00 (CET)

    Overview:

    In this webinar, you will learn about a novel extraction method for analyzing PFAS in lipid-rich, full-fish homogenates, addressing challenges with EPA Method 1633. Traditional methods struggle with complex matrices like whole fish, often leading to incomplete PFAS separation. Our method achieves recoveries of 73-104% for linear perfluoro carboxylic and perfluoro sulfonic acids, with improved detection in Pacific Herring and Chinook salmon samples. This approach offers more accurate trophic magnification analysis and can be applied to archived fish samples, enhancing historical PFAS research.

    Key Learning Points:

    • Understand the challenges of analyzing PFAS in lipid-rich, full-fish homogenates and the limitations of EPA Method 1633
    • Learn about a new extraction method that improves PFAS recovery and detection in complex biological matrices
    • Explore how this method enhances trophic magnification analysis and supports historical PFAS research using archived fish samples

    Who should attend?

    Any scientists and researchers interested or conducting PFAS analysis that need to avoid matrix interferences.

    Guest Speaker: Sydney Brady Ph.D., Candidate Indiana University O’Neill School of Public and Environmental Affairs. Bloomington, IN


    Symbiosis of Core-Shell and Fully Porous Particles

    Date: 28 April 2025

    Time: 10:00 - 11:00 (GMT) | 11:00 - 12:00 (CET)

    Key Learning Points:

    • Find how best to utilize modern UHPLC particles to optimize chromatographic performance
    • Learn when to consider particle morphologies as a method development tool
    • Learn when to consider phase selectivity as a method development tool

    Who should attend?

    Any LC User who is interested in the performance benefits, and differences, of both core- shell and full porous particles.

    Presenter: Sean Orlowicz, Principal Market Development Manager - Pharmaceutical, Phenomenex


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