Introduction to HPLC

(Course No. SS0-9377)

Date and Location
6-7 May 2025
or 29-30 September 2025
08:30 - 12:30 GMT | 09:30 - 13:30 CET
Online

Please note: the seminars take place in two half days (2 sessions of 4 hours each).

Price (per person)
£425 | 485 € | 480 CHF | 1200 PLN | 3600 DKK | 5100 NOK | 5350 SEK | 9300 CZK (+VAT, incl. PDF of the slides and certificate of attendance)

Course Summary

Learn how to set up and run HPLC analysis with a full understanding of all the chromatography theory and method parameters, such as the column, the mobile phase, the instrumentation, and sample preparation. A practical section reporting a detailed discussion about measurement, method and a real case study is also included.
This course is ideal for those who are new to HPLC.

Course Outline

Instrumentation

  • Full system, pump, injector
  • Column and column heater
  • Detectors overview

Theory

  • Mobile phase
  • Separation modes
  • Columns and stationary phase
  • Chromatography theory

Practical Section

  • Measurements
  • Methods
  • Real case study

Practical skills acquired

This course will enable you to implement HPLC analytical methods by a detailed description of HPLC parameters. In addition you will be able to:

    • Understand what is meant by all the parameters in an HPLC analytical method.
    • Follow an HPLC analytical method to set up an HPLC system for analysis.
    • Run an HPLC analytical method and acquire chromatographic results.
    • Interpret chromatograms obtained from HPLC analysis.

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