Introduction to Biochromatography Seminar

Date and Location
On site or Online - on demand

Course Summary

In this basic seminar we provide a comprehensive overview of typical workflows and methods for the characterisation of proteins and peptides. We give valuable tips for sample preparation and discuss the most important optimisation parameters for chromatographic separations using size exclusion, ion exchange, reversed phase or HILIC chromatography.

Course Outline

Basics about peptides, proteins and antibodies

Aggregate and fragment analysis of mAbs and other biologics using size exclusion chromatography

Characterisation of antibody charge variants by ion exchange chromatography and optimisation of salt and pH gradients

Use of widepore core-shell particles for the RP analysis of intact biologics and their subunits

Improvement of resolution and peak shape in peptide mapping and peptide quantification

Enzymatic digestion and reduction of proteins

Sample preparation using magnetic beads

Separation of released and labelled glycans using HILIC chromatography

Practical Skills Acquired

With the background knowledge gained, you should be able to develop and optimise a method for biologics faster and in a more targeted manner in the future by:

Minimising undesirable secondary interactions, problematic carryover and recovery problems through the use of biocompatible hardware components.
Expanding the experimental design space for different separation techniques and optimising key method parameters for both UV and MS detection.
Using the latest column technologies to adjust selectivity according to your separation goal.

Is this seminar of interest to your lab? Please contact your local Technical Sales Consultant or email phenomenexeu@phenomenex.com to request a seminar.

Subject to Phenomenex terms and conditions chevron_right

Have a question about these seminars? Please contact us

UK/IE Office and International

UK: 01625 501367
IE: 01 247 5405
Int: +44 1625 501367
Email Us ›

Nordic Office

DK: 4824 8048 | NO: +47 810 02 005
SE: 08 611 6950 | FI: +358 (0)9 4789 0063
Email Us ›

BeneLux Office

NL/BE: +31 (0)30-2418700
Email Us ›

Swiss Office

CH: +49 (0)6021-58830-0
Email Us ›

Polish Office

PL: + 48 22 510 21 80
Email Us ›

Czech Office

CZ: +420 272 017 077
Email Us ›