Introduction to Biochromatography Seminar

Date and Location
On demand - Online

Please note: the seminar takes place on two half days (2 sessions of 4 hours each). Minimum of 4 attendees.

Price (per person)
£425 | 485 € | 480 CHF | 1200 PLN | 3600 DKK | 5100 NOK | 5350 SEK | 9300 CZK (+VAT, incl. PDF of the slides and certificate of attendance)

Course Summary

In this basic seminar we provide a comprehensive overview of typical workflows and methods for the characterisation of proteins and peptides. We give valuable tips for sample preparation and discuss the most important optimisation parameters for chromatographic separations using size exclusion, ion exchange, reversed phase or HILIC chromatography.

Course Outline

Basics about peptides, proteins and antibodies

Aggregate and fragment analysis of mAbs and other biologics using size exclusion chromatography

Characterisation of antibody charge variants by ion exchange chromatography and optimisation of salt and pH gradients

Use of widepore core-shell particles for the RP analysis of intact biologics and their subunits

Improvement of resolution and peak shape in peptide mapping and peptide quantification

Enzymatic digestion and reduction of proteins

Sample preparation using magnetic beads

Separation of released and labelled glycans using HILIC chromatography

Practical Skills Acquired

With the background knowledge gained, you should be able to develop and optimise a method for biologics faster and in a more targeted manner in the future by:

Minimising undesirable secondary interactions, problematic carryover and recovery problems through the use of biocompatible hardware components.
Expanding the experimental design space for different separation techniques and optimising key method parameters for both UV and MS detection.
Using the latest column technologies to adjust selectivity according to your separation goal.

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