An Introduction to LC-MS

Part I. Intro to Liquid Chromatography

• What is the role of the LC column in LC-MS?
• Why is it even necessary?
• Basics of LC theory
• Review of the different LC media/support particles
• Survey of different LC bonded phases and their application
• Guide to selecting the right LC column for your application
• Effect of temperature
• Solvent selection
• HPLC/UHPLC column care

Part II. Introduction to LC-MS

Led by an Application Specialist from SCIEX this module will introduce and develop new practitioners by delivering an understanding of basic mechanisms associated with LC-MS/MS. As well as discussing mass spectrometry fundamentals, the material will then go on to examine the most commonly applied LC-MS techniques and the principles behind them:

Atmospheric Pressure Ionisation theory, techniques and methods:

• Desolvation
• Electrospray Ionisation (ESI)
• Atmospheric Chemical Ionisation (APCI)

Mass Analysis

• The importance of calibration
• The differences between nominal mass, accurate mass and mass resolution

Mass Analysers

• Quadrupole – benefits, limitations and general usage
• Time of Flight – benefits, limitations and general usage

Data analysis

• Quantitation
• Application of targeted techniques eg MRM to data acquisition and processing
• Qualitative approaches to identification
• Application of discovery techniques eg scanning, information dependent acquisition, library searching