An Introduction to LC-MS

Part I. Introduction to Mass Spectrometry:

This module will introduce and develop new practitioners by delivering an understanding of basic mechanisms associated with LC-MS. As well as discussing mass spectrometry fundamentals, the material will then go on to examine the most commonly applied LC-MS techniques and the principles behind them:

• Atmospheric pressure ionisation theory
  • Desolvation
  • Electrospray Ionisation (ESI)
  • Atmospheric Chemical Ionisation (APCI)
• Mass spectrometry definition
  • Overview of the key definitions in mass spectrometry; mass accuracy and resolution, nominal versus accurate mass and isotopic pattern identification
• Mass analyser: benefits, limitations and general usage
  • Quadrupole
  • Ion trap
  • Time of flight
  • Orbitrap
• Mass spectrometry workflow
  • Acquisition procedures
  • Qualitative and quantitative approach
  • Case studies

Part II. Liquid Chromatography for Mass Spectrometry:

• What is the role of the LC column in LC-MS?
• Why is it even necessary?
• Basics of LC theory
• Review of the different LC media/support particles
• Mobile phases and buffer choice for LC-MS
• Common contaminants with MS