FREE WEBINARS

 
Selectivity for Bioanalytical LC Methods: A Practical Approach to Column Selection
Kevin Zhao
Speaker:
Kevin Zhao
Global Technical Manager, Phenomenex

Hui (Kevin) Zhao is a Global Technical Manager at Phenomenex, a Danaher company, with more than 13 years of experience in chromatography, sample preparation, analytical method development, and technical support. Kevin provides global technical leadership and works closely with customers and cross-functional teams to solve complex chromatography and product-performance challenges. His technical expertise includes HPLC, UHPLC, LC-MS/MS, GC, GC-MS, SPE, method development, and chromatographic troubleshooting, with experience supporting pharmaceutical, biopharmaceutical, clinical, environmental, and food-safety applications. Kevin also develops and delivers technical training, webinars, and customer seminars globally. He holds an M.S. in Biochemistry and a B.S. in Biomedical Science.

One webinar, two viewing options
We know you're busy, select a viewing session that works best for you.
Viewing Options
Session 1
Tuesday, December 8, 2026
Los Angeles: 10:00 AM PT | New York: 1:00 PM EST
Mexico City: 12:00 PM CDT

Session 2
Wednesday, December 9, 2026
London: 9:00 AM GMT | Berlin: 10:00 AM UTC +1 Mumbai: 1:30 PM IST
Overview
This session will cover why stationary-phase selectivity is a critical starting point for small-molecule bioanalytical LC and LC-MS method development, and how analyte properties and bio-sample matrix challenges influence reversed-phase column selection. It will show how to use a complementary column-screening set to compare C18 and alternative selectivities efficiently, and how chromatographic selectivity, retention, peak shape, and separation from matrix components support reproducible and robust quantitative and qualitative analysis. It will also address practical troubleshooting considerations for when an initial column does not provide adequate retention, resolution, or peak shape.
Key Learning Points
  • Learn how analyte chemistry and stationary-phase interactions affect LC selectivity.
  • Select a practical starting column and a complementary method-development column set for small-molecule DMPK or TDM applications.
  • Plan an efficient column-screening workflow using orthogonal selectivities rather than relying on a single C18 phase.
  • Evaluate candidate conditions using retention, resolution, peak shape, matrix separation, and LC-MS compatibility.
  • Apply column-selection decisions to improve method reproducibility and robustness.
Who Should Attend
  • LC and LC-MS scientists, bioanalytical researchers, and laboratory professionals working with small-molecule DMPK or bio-sample Therapeutic Drug Monitoring (TDM) applications who want practical guidance on column selection and method development to build more reproducible, robust, and reliable methods.
PhenoAcademy




I would like to receive personalized information about products and services from Phenomenex and other Danaher group companies as indicated below. I understand that my preferences and interactions, including whether I’ve opened your messages, will be used to personalize content to my interests.
By submitting your information, you agree that Phenomenex may collect and further process your personal data in accordance with its Privacy Policy.
You may object to receiving further information at any time.