Join Our FREE On-Demand Webinar!

Sustainable Strategies for High Sensitivity Analysis for Clinical Research LC-MS/MS Methods

Supported Liquid Extraction (SLE) for the cleanup of biological matrices has several advantages over Solid Phase Extraction (SPE). SLE requires fewer steps and less method development compared to SPE. For most samples that can be processed by liquid-liquid extraction, SLE can usually be used to provide cleaner and faster solvent partitioning. Historically, to get the cleanest SLE extracts, halogenated solvents like dichloromethane were used for elution of target analytes from the sorbent. A supported liquid extraction (SLE) approach that produced clean extracts with high recoveries and low matrix interferences using ethyl acetate, a more sustainable organic solvent, will be presented. Results for steroids, methylmalonic acid (MMA), and underivatized estrogens in human serum, in addition to optimized LC-MS/MS conditions and LC column considerations, are also discussed.

First Name*

Last Name*

Country*

Company*

Company Email*

We'd love to keep in touch with solutions that may be of interest to you. Please check the box(es) below to confirm how you prefer Phenomenex or our affiliates to contact you.

By Email

By Phone

By submitting your information, you agree that Phenomenex may collect and further process your personal data in accordance with its Privacy Policy.