HOW TO DEVELOP HPLC METHODS

REGISTER FOR OUR TRAINING SEMINAR!

HOW TO DEVELOP HPLC METHODS

Genevieve Hodson

Manager of Technical Support, Phenomenex

September 27 - September 28, 2023

8:00 AM - 12:00 PM (PT)

Cost Per Person: $300

Location: Online

Limited Capacity

Course Agenda:

Part 1 – Analytes and Interactions

LogP, dissolution, pKa, interactions with stationary phase, and separation modes

Part 2 – Column Dimensions

Column ID, length, particle size, hardware, and limitations

Part 3 – Stationary Phase

Selectivity, interactions, and special phases (high pH, polymer, aqueous stable)

Part 4 – Mobile Phase

Selectivities of organics, buffer selection (pH), and gradient slope

Part 5 – Other Parameters

Flow rate, temperature, connections, injection programs, and sample diluent

Key Learning Objectives:

Understand how to select column stationary phase for LC analysis, and how to choose mobile phase to get better resolution
Learn different separation modes used in Liquid Chromatography
Review parameters that can be adjusted during LC method development

Who Should Attended:

This course is ideal for any HPLC analyst who is new to chromatography and would like to save time on column selection, or a better understanding

Speaker Bio:

Genevieve graduated from the University of Texas with a BS in Chemistry located in her home town of Austin Texas. She spent 2 years of her undergraduate performing research in an Organic Chemistry Lab synthesizing intermediates. Out of school she spent 4 years as an Analytical Chemist in the QC and R&D Department of Cerilliant (a Sigma Millipore Company). There she worked with many analytical instruments but became an expert at LC by performing purity analysis, customer specific methods, validations, qc testing and stability studies on a wide range of small molecules including pharmaceuticals, pesticides, explosives and illegal substances. From there she moved to Los Angeles California where she worked at Johnson and Johnson and then a small dietary supplement company. After Genevieve spent a year living abroad in Israel raising her first son, she moved back to LA where she got hired as a Technical Specialist at Phenomenex. At Phenomenex she enjoys continuing to learn about chromatography every day through helping customer with all their chromatography needs!

Please email any questions to events@phenomenex.com.

For cancellations made 10 days before the date of the course, the full amount will be refunded. No refunds will be issued for cancellations made after this deadline. It will be possible to replace a participant with another name upon written notice.

Seminar Details

General Information

Each seminar will take place from 8:00 AM - 12 PM PT over the course of two days. The seminars will be delivered online via our training platform; GotoTraining, with each registrant receiving their log-in link starting eight days before the seminar starts. The seminars will NOT be recorded so you must be able to attended during the scheduled times in order to benefit from these courses.

All registrants will be mailed the printed presentation before the seminar begins.
We do expect each seminar to sell out, so register now to secure your seat!
Bring your questions to the seminar, our trainers will be ready to help.
View seminar Terms and Conditions.