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Eliminating Matrix Effects in Lipid-rich, Full-Fish Homogenates
PhenoWebinars
Guest Speaker: Sydney Brady Ph.D. 
Candidate Indiana University O’Neill School of Public and Environmental Affairs. Bloomington, IN
Tuesday, April 15, 2025
10:00 AM (PT)
Overview
In this webinar, you will learn about a novel extraction method for analyzing PFAS in lipid-rich, full-fish homogenates, addressing challenges with EPA Method 1633. Traditional methods struggle with complex matrices like whole fish, often leading to incomplete PFAS separation. Our method achieves recoveries of 73-104% for linear perfluoro carboxylic and perfluoro sulfonic acids, with improved detection in Pacific Herring and Chinook salmon samples. This approach offers more accurate trophic magnification analysis and can be applied to archived fish samples, enhancing historical PFAS research.
Key Learning Points
  • Understand the challenges of analyzing PFAS in lipid-rich, full-fish homogenates and the limitations of EPA Method 1633.
  • Learn about a new extraction method that improves PFAS recovery and detection in complex biological matrices.
  • Explore how this method enhances trophic magnification analysis and supports historical PFAS research using archived fish samples.
Who Should Attend
  • Any scientists and researchers interested or conducting PFAS analysis that need to avoid matrix interferences.
Speaker Bio
Sydney Brady, Ph.D., is an environmental scientist focusing on the fate and transport of chemical pollution in biotic and abiotic matrices. She is currently a PhD candidate at Indiana University. Her research focuses on developing methods to measure PFAS in complex matrices and determining the fate and transport of PFAS and legacy organic pollutants in avian sentinel species."
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