LC TIPS

Fundamental​

• The importance of a well-prepared mobile phase →
• The effects of gradient on buffer capacity  →
• How can capacity factors drive method robustness?  →
• The role of end-capping in RP →
• Overcoming Peak Tailing of Basic Analytes in Silica Type A Stationary Phases in RP  →
• NEW Basic analytes and the benefits of modified silica supports in reversed-phase LC  →

Method Development​

• A systematic approach to chiral screening and method development
• Advantages of using immobilized stationary phases in chiral separations →
• More coming soon

Troubleshooting​

• Column contamination – what is happening, and how it can be avoided →
• The danger of mobile phase mismatch →

GC TIPS

Fundamental​

• Pre-installation checklist and how to install new GC column → 
• Gas Chromatography Column Installation - Detector Installation →
• Gas Chromatography (GC) Column Conditioning, Testing and Checks →

Method Development​

• What parameters should I consider when selecting a GC column for my application? →
• GC Column Selection by Column Dimension →
• Good column selection: Polarity vs Selectivity →

Troubleshooting​

• Peak Shape Problems: Tailing Peaks 
• Peak Shape Problems: Sensitivity Loss →
• Peak Shape Problems: Broad solvent Peaks/fronts →
• NEW Peak Shape Problems: No Peaks →

SAMPLE PREP TIPS

Fundamental​

• Generic SPE Method by Retention Mechanism →
• How is normal phase SPE different from reversed phase SPE? How do I decide which one to use?  →
• How much sample can I load onto my SPE device? Is it linked to the concentration of my analyte of interest? →

Application

• Considerations when working with solid samples and SPE →
• Sample Pre-treatment Procedures for Bioanalytical Samples →
• Peptide Mapping Sample Prep - Good Practices for Tryptic Digests →
• More coming soon

Troubleshooting​

• Over-Drying of Silica-Based SPE Cartridges Can Lead to Poor Sample Recovery
• Troubleshooting SPE  →
• NEW I am getting a low recovery from my SPE method, how do I fix the problem? →