Course No:SS0-9360
An Introduction to LC-MS
- Full day comprehensive course from 09:00 - 16:30
- All course materials will be provided
Locations and Dates: Online Training - 04.11.2020
Registration Cost: Price: £79 + VAT
Part I. Intro to Liquid Chromatography:
- What is the role of the LC column in LC-MS?
- Why is it even necessary?
- Basics of LC theory
- Review of the different LC media/support particles
- Survey of different LC bonded phases and their application
- Guide to selecting the right LC column for your application
- Effect of temperature
- Solvent selection
- HPLC/UHPLC column care
Part II. Introduction to LC-MS :
Led by an Application Specialist from SCIEX this module will introduce and develop new practitioners by delivering an understanding of basic mechanisms associated with LC-MS/MS. As well as discussing mass spectrometry fundamentals, the material will then go on to examine the most commonly applied LC-MS techniques and the principles behind them:
- Atmospheric Pressure Ionisation theory, techniques and methods:
- Desolvation
- Electrospray Ionisation (ESI)
- Atmospheric Chemical Ionisation (APCI)
- Mass Analysis
- The importance of calibration, and the differences between nominal mass, accurate mass and mass resolution
- Mass Analysers
- Quadrupole – benefits, limitations and general usage
- Time of Flight – benefits, limitations and general usage
- Data analysis
- Quantitation
- Application of targeted techniques eg MRM to data acquisition and processing
- Qualitative approaches to identification
- Application of discovery techniques eg scanning, information dependent acquisition, library searching
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