LC Method Development Training Course (No:SS0-5946):
How to Develop HPLC Methods for Challenging Separations
  • Full day comprehensive course from 09:00 - 16:30
  • Lunch and Refreshments will be provided
  • All course materials will be provided
Learn how to implement strategies to achieve satisfactory separation for ‘complex’ samples and use computer modelling to develop robust and fit for purpose HPLC methods. This one day course is ideal for those who have experience developing HPLC methods but want to increase their knowledge to deal with more challenging separations. Although designed as a follow-up to the course “How to Develop HPLC Methods”, it is not a prerequisite. This course is suitable for learners who feel confident that they are familiar with all the content detailed for the course “How to Develop HPLC Methods”. 

Practical skills acquired
This course will enable you to find solutions for difficult HPLC separations. In addition you will be able to:

  • Understand why some separations can be challenging
  • Identify potential problem separations.
Apply strategies to achieve satisfactory separations for “complex” samples with respect to:
  • Sample preparation
  • Detection methods
  • Retaining very polar analytes
  • Optimising gradient methods
  • Selecting columns
  • Setting up scouting/screening experiments
Course Outline
Implementing a 5-step strategy for method development of complex samples
Review of a 5-step strategy for HPLC method development with particular attention to reasons why some separations are‘challenging’:

  • Setting suitable objectives for method development
  • Assessing all available information
  • Selecting suitable samples
  • Performing scouting experiments to select suitable initial conditions
  • Optimising the method to define method parameters which achieve the desired separation.

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Attendee Details

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Locations and Dates
London - 29.01.2020

Manchester - 08.07.2020

Dublin - 15.10.2020

London - 18.11.2020
Registration Cost
Price: £285 + VAT

Current Offers
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Promotion Code: TCMP20
 
Alternatively:
Tel: (UK) 01625 501367 | (Ireland) 01 247 5405 | (International) +44 1625 501367 | Email: ukinfo@phenomenex.com

Seminar Locations
London:
Grand Connaught Rooms
61-63 Great Queens Street,
London
WC2B 5DA
Manchester:
The Midland Hotel
16 Peter St,
Manchester,
M60 2DS
Leeds:
The Met Hotel Leeds
King Street,
Leeds,
LS1 2HQ
Dublin:
Louis Fitzgerald Hotel
Newland's Cross,
Naas Road,
Dublin
Glasgow:
Biocity,
Bo'Ness Rd, Newhouse, Chapelhall,
Motherwell,
ML1 5UH
Bristol:
DoubleTree by Hilton Bristol City Centre
Redcliffe Way,
Bristol,
BS1 6NJ
Cambridge:
DoubleTree by Hilton Hotel
Granta Place,
Mill Ln, Cambridge,
CB2 1RT
Newcastle:
Copthorne Hotel
The Close,
Quayside, Newcastle upon Tyne,
NE1 3RT

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