This course is split over 2 half-days. Registration cost covers both dates.
Date and Location
17 and 18 September 2024
08:30 - 12:30 BST | 09:30 - 13:30 CEST
Online
Price (per person)
£425 | 485 € | 480 CHF | 1200 PLN | 3600 DKK | 5100 NOK | 5350 SEK | 9300 CZK (+VAT, incl. PDF of the slides and certificate of attendance)
Course Summary
In this training we will discuss sample preparation and good practices that can impact the quality of your result from peptide mapping depending on your Lab’s analytical scope. The course will explore and present specific examples in Sample Preparation and Chromatography.
Course Outline
Part 1: Peptide overview
- Digestion of Proteins
- Chromatographic Selectivity
- Method Development for Peptide Analysis
Part 2: Peptide overview - cont.
- PTM Identification
- Sample preparation using Magbeads
- Analytical vs. Nano flow
Part 3: Case Studies
- Liraglutide
- Bivalirudin
Part 4: Good Laboratory Practices
Practical Skills Acquired
This course will enable you to find solutions to your Peptide Mapping challenges. In addition, you will be able to:
- Drive a chromatographic optimization for peptide mapping, including investigating differences in method parameters, designing effective gradient programs, column selecting the best particle/phase selection, among others
- Recognize the most suitable acid modifier depending on the context of the analytical method
- Choose the correct chromatography conditions and selectivity to achieve the best peak capacity and largest number of peptide/protein identifications