Date and Location
On site or Online - on demand
Course Summary
In this course we will discuss sample preparation and good practices that can impact the quality of your result from peptide mapping depending on your lab’s analytical scope. The course will explore and present specific examples in sample preparation and chromatography.
Course Outline
Part 1: Peptide overview
- Digestion of Proteins
- Chromatographic Selectivity
- Method development for Peptide Analysis
Part 2: Peptide overview - cont.
- PTM Identification
- Sample preparation using Magbeads
- Analytical vs. Nano flow
Part 3: Case Studies
- Signature peptides using MagBeads
Part 4: Good Laboratory Practices
Practical Skills Acquired
This course will enable you to find solutions to your peptide mapping challenges. In addition, you will be able to:
- Drive a chromatographic optimisation for peptide mapping, including investigating differences in method parameters, designing effective gradient programs, column selection, selecting the best particle/phase selection, among others.
- Recognise the most suitable acid modifier depending on the context of the analytical method.
- Choose the correct chromatography conditions and selectivity to achieve the best peak capacity and largest number of peptide/protein identifications.
Is this seminar of interest to your lab? Please contact your local Technical Sales
Consultant or email phenomenexeu@phenomenex.com to request a seminar.
Have a question about these seminars? Please contact us
Nordic Office
DK: 4824 8048 | NO: +47 810 02 005SE: 08 611 6950 | FI: +358 (0)9 4789 0063
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