Technical Poster Request/ASMS

mRNA Oligonucleotide Characterization via Low Flow Ion Pair-Reversed Phase Chromatography and Tandem Mass Spectrometry (LF-IP-RP-MS/MS)

Poster Abstract:

mRNA is a rapidly growing therapeutic modality with numerous drug candidates in the pipeline globally. The manufacturing process of mRNA molecules consists of in vitro transcription reactions in which the desired final product is over 1000 nucleotides in length, has a 5’ Cap, and a Poly(A) tail. Incomplete capping and Poly(A) length variations can be present in the final product and need to be quantified to assess purity. Analysis of mRNA via LC-MS can e a challenge due to low amounts of sample. Decreasing column inner diameter increases sensitivity consequently improving mass spectra quality and oligo identification. Here we show a reliable workflow for the characterization and quantitation of mRNA therapeutics using

enzymatic digestion followed by LF-IP-RPLC-MS/MS.

mRNA Oligonucleotide Characterization via Low Flow Ion Pair-Reversed Phase Chromatography and Tandem Mass Spectrometry (LF-IP-RP-MS/MS)

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