Technical Poster Request/ASMS

Improved Intact Protein LC-MS Workflows for the Analysis and Quantitation of Adeno-Associated Viral Capsids

Poster Abstract:

Adeno-associated viruses (AAVs) are widely used vectors for gene therapy development. The AAV capsid is composed of 3 viral proteins that share high sequence homology among stereotypes. Different production methods can result in different expression levels of capsid proteins. Also, some post-translational modifications (PTMs) can affect potency and infectivity. Thus, AAV capsid protein quantitation is crucial for determining product quality and consistency (protein ratios). In this study, reversed-phase liquid chromatography coupled with high resolution mass spectrometry was used to identify and relatively quantify capsid proteins across different variants This work also evaluates the effects of using of various mobile phase modifiers as well as sample denaturation methods to achieve complete chromatographic separation of AAV capsid proteins for optimal HRMS analysis.

Improved Intact Protein LC-MS Workflows for the Analysis and Quantitation of Adeno-Associated Viral Capsids

Request a Copy of this Poster Presented at ASMS 2024

Please select how we can help you?*

Yes, contact me for pricing, a quote or further information

No, thanks

Please provide more details about your request...

Email Address*

First Name*

Last Name*

Company*

Country*

We'd love to keep in touch with solutions that may be of interest to you. Please check the box(es) below to confirm how you prefer Phenomenex to contact you.

By Email

By Phone