Fast Cleanup for 19 Steroid Analytes in Serum Using Supported Liquid Extraction (SLE)

Poster Abstract

Steroid analysis for clinical research can require very low limits of detection which demand high recoveries and very clean

extracted samples. Supported Liquid Extraction (SLE) can provide very clean extracted samples with less method

development and a streamlined workflow compared to solid phase extraction (SPE). Laboratories desire high-throughput

methods which consolidate analytes into one panel with fast chromatographic run times. Chromatographic separation of

steroid isomers with the same m/z is necessary for accurate quantitation by LC-MS/MS. Meeting these criteria can be

challenging in a single LC-MS/MS method.

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