Sample Preparation Technical Tip

Sample Preparation

Method and Sorbent Volume Selection

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The volume of solvent needed for SPE processing is directly related to the mass of sorbent in the SPE tube and more specifically the “bed volume” of the SPE device. Intuitively we know more sorbent requires more solvent, less sorbent = less solvent. Typically 4 – 16 bed volumes are used in SPE methods.

Sorbent Wash and Elution Volumes*

Silica-Based Sorbent Mass	Practical Minimum Wash and Elution Volume 4 bed volumes	Recommended Wash and Elution Volume 8 bed volumes	Polymer-Based Sorbent Mass*	Practical Minimum Wash and Elution Volume 4 bed volumes	Recommended Wash and Elution Volume 8 bed volumes
10 mg	60 μL	120 μL	10 mg	100 μL	200 μL
-	-	-	30 mg	300 μL	600 μL
50 mg	300 μL	600 μL	-	-	-
-	-	-	60 mg	600 μL	1.2 mL
100 mg	600 μL	1.2 mL	100 mg	1 mL	2 mL
150 mg	900 μL	1.8 mL	150 mg	1.5 mL	3 mL
200 mg	1.2 mL	2.4 mL	200 mg	2 mL	4 mL
500 mg	3 mL	6 mL	500 mg	5 mL	10 mL
1 g	6 mL	12 mL	1 g	10 mL	20 mL
2 g	12 mL	24 mL	-	-	-
5 g	30 mL	60 mL	-	-	-
10 g	60 mL	120 mL	-	-	-

*Strata-X polymeric resins have a larger surface area than Strata silica based material, hence requiring slightly more solvent per gram for processing. The elution volumes are specific to the chemical nature of the analyte being extracted, its concentration in the sample, the chemical nature of the eluting solvent and the bed mass used. The above is a guideline. An elution study should be conducted to determine the appropriate volume to use.

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