Sensitivity Loss: Some or all of your peaks are displaying decreased response. What is going on and how can you remedy this issue?
Symptom | Possible Cause | Suggested Remedy |
---|---|---|
Decreased Response | Contamination of column and/ or liner can lead to loss of sensitivity for active compounds. | Clean liner.
See injector maintenance.
Bake out the column. Solvent rinse or replace the column. See column installation. |
Injector leaks reduce the peak height of the most volatile components of a sample. | Find and fix any leaks. | |
If initial column temperature is too high for splitless injection, this prevents refocusing of sample. This affects the more volatile components most. | Lower temperature below the boiling point of the solvent. Decrease the initial column temperature, or use a less volatile solvent | |
Inlet discrimination: injector temperature is too low. Later eluting and less volatile compounds have low response. | Increase the injection temperature or use on-column injection with direct connect liner. | |
Issues with the sample | Check the sample concentration, any sample preparation procedures, and shelf life. Prepare a fresh sample ensuring the proper concentration. |
GC Column Selection and Gas Management Poster
Side 1: Tips and Steps for selecting the correct Gas Management Filters for your GC or LC system; And Inline Gas Traps for your GC or GC-MS system.
- Side 2: Guidelines for your GC column selection process:
- The Master Resolution Equation
- Internal Diameter
- Film Thickness
- Length
- Column Phase